Surface motion upregulates superficial zone protein and hyaluronan production in chondrocyte-seeded three-dimensional scaffolds.

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  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9505538 Publication Model: Print Cited Medium: Print ISSN: 1076-3279 (Print) Linking ISSN: 10763279 NLM ISO Abbreviation: Tissue Eng Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, NY : Mary Ann Liebert, Inc., c1995-
    • Subject Terms:
      Motion* ; Up-Regulation*; Cell Culture Techniques/*instrumentation ; Chondrocytes/*physiology ; Hyaluronic Acid/*biosynthesis ; Proteins/*metabolism; Animals ; Bioreactors ; Cartilage, Articular/cytology ; Cattle ; Cell Culture Techniques/methods ; Cells, Cultured ; Chondrocytes/cytology ; Chondrocytes/drug effects ; Culture Media, Conditioned ; Gene Expression Regulation/drug effects ; Joints/cytology ; Polyurethanes/pharmacology ; RNA, Messenger/analysis ; RNA, Messenger/metabolism ; Stress, Mechanical
    • Abstract:
      A cartilage engineering bioreactor has been developed that provides joint-specific kinematics. This study investigated the effect of articular motion on the gene expression of superficial zone protein (SZP) and hyaluronan synthases (HASs) and on the release of SZP and hyaluronan of chondrocytes seeded onto biodegradable scaffolds. Cylindrical (8 x 4 mm) porous polyurethane scaffolds were seeded with bovine articular chondrocytes and subjected to static or dynamic compression, with and without articulation against a ceramic hip ball. After loading, the mRNA expression of SZP and HASs was analyzed, and SZP immunoreactivity and hyaluronan concentration of conditioned media were determined. Surface motion significantly upregulated the mRNA expression of SZP and HASs. Axial compression alone had no effect on SZP and increased HAS mRNA only at high strain amplitude. SZP was immunodetected only in the media of constructs exposed to surface motion. The release of hyaluronan into the culture medium was significantly enhanced by surface motion. These results indicate that specific stimuli that mimic the kinematics of natural joints, such as articular motion, may promote the development of a functional articular surface-synovial interface.
    • Accession Number:
      0 (Culture Media, Conditioned)
      0 (Polyurethanes)
      0 (Proteins)
      0 (RNA, Messenger)
      9004-61-9 (Hyaluronic Acid)
    • Publication Date:
      Date Created: 20050302 Date Completed: 20050623 Latest Revision: 20220316
    • Publication Date:
      20221213
    • Accession Number:
      10.1089/ten.2005.11.249
    • Accession Number:
      15738679