Adaptive translocation: the role of hydrogen bonding and membrane potential in the uptake of guanidinium-rich transporters into cells.

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  • Author(s): Rothbard JB;Rothbard JB; Jessop TC; Wender PA
  • Source:
    Advanced drug delivery reviews [Adv Drug Deliv Rev] 2005 Feb 28; Vol. 57 (4), pp. 495-504. Date of Electronic Publication: 2005 Jan 11.
  • Publication Type:
    Journal Article; Review
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Science Publishers, B.V Country of Publication: Netherlands NLM ID: 8710523 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0169-409X (Print) Linking ISSN: 0169409X NLM ISO Abbreviation: Adv Drug Deliv Rev Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier Science Publishers, B.V., c1987-
    • Subject Terms:
      Hydrogen Bonding*; Endocytosis/*physiology ; Guanidine/*metabolism ; Membrane Transport Proteins/*metabolism; Biological Transport ; Guanidine/chemistry ; Humans ; Membrane Potentials ; Membrane Transport Proteins/chemistry
    • Abstract:
      A mechanistic hypothesis is presented for how water-soluble guanidinium-rich transporters attached to small cargoes (MW ca. <3000) can migrate across the non-polar lipid membrane of a cell and enter the cytosol. Positively charged and water-soluble, arginine oligomers can associate with negatively charged, bidentate hydrogen bond acceptor groups of endogenous membrane constituents, leading to the formation of membrane-soluble ion pair complexes. The resultant less polar, ion pair complexes partition into the lipid bilayer and migrate in a direction, and with a rate, influenced by the membrane potential. The complex dissociates on the inner leaf of the membrane and the transporter conjugate enters the cytosol. This mechanism could also be involved in the translocation of guanidinium-rich molecules that are endocytosed due to their size or the conditions of the assay, across the endosomal membrane.
    • Number of References:
      35
    • Accession Number:
      0 (Membrane Transport Proteins)
      JU58VJ6Y3B (Guanidine)
    • Publication Date:
      Date Created: 20050222 Date Completed: 20050524 Latest Revision: 20131121
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.addr.2004.10.003
    • Accession Number:
      15722160