Menu
×
West Ashley Library
Closed
Phone: (843) 766-6635
Folly Beach Library
Closed
Phone: (843) 588-2001
Edgar Allan Poe/Sullivan's Island Library
Closed for renovations
Phone: (843) 883-3914
Wando Mount Pleasant Library
Closed
Phone: (843) 805-6888
Village Library
Closed
Phone: (843) 884-9741
St. Paul's/Hollywood Library
Closed
Phone: (843) 889-3300
Otranto Road Library
Closed
Phone: (843) 572-4094
Mt. Pleasant Library
Closed
Phone: (843) 849-6161
McClellanville Library
Closed
Phone: (843) 887-3699
Keith Summey North Charleston Library
Closed
Phone: (843) 744-2489
John's Island Library
Closed
Phone: (843) 559-1945
Hurd/St. Andrews Library
Closed
Phone: (843) 766-2546
Miss Jane's Building (Edisto Library Temporary Location)
Closed
Phone: (843) 869-2355
Dorchester Road Library
Closed
Phone: (843) 552-6466
John L. Dart Library
Closed
Phone: (843) 722-7550
Baxter-Patrick James Island
Closed
Phone: (843) 795-6679
Main Library
2 p.m. – 5 p.m.
Phone: (843) 805-6930
Bees Ferry West Ashley Library
Closed
Phone: (843) 805-6892
Mobile Library
Closed
Phone: (843) 805-6909
Today's Hours
West Ashley Library
Closed
Phone: (843) 766-6635
Folly Beach Library
Closed
Phone: (843) 588-2001
Edgar Allan Poe/Sullivan's Island Library
Closed for renovations
Phone: (843) 883-3914
Wando Mount Pleasant Library
Closed
Phone: (843) 805-6888
Village Library
Closed
Phone: (843) 884-9741
St. Paul's/Hollywood Library
Closed
Phone: (843) 889-3300
Otranto Road Library
Closed
Phone: (843) 572-4094
Mt. Pleasant Library
Closed
Phone: (843) 849-6161
McClellanville Library
Closed
Phone: (843) 887-3699
Keith Summey North Charleston Library
Closed
Phone: (843) 744-2489
John's Island Library
Closed
Phone: (843) 559-1945
Hurd/St. Andrews Library
Closed
Phone: (843) 766-2546
Miss Jane's Building (Edisto Library Temporary Location)
Closed
Phone: (843) 869-2355
Dorchester Road Library
Closed
Phone: (843) 552-6466
John L. Dart Library
Closed
Phone: (843) 722-7550
Baxter-Patrick James Island
Closed
Phone: (843) 795-6679
Main Library
2 p.m. – 5 p.m.
Phone: (843) 805-6930
Bees Ferry West Ashley Library
Closed
Phone: (843) 805-6892
Mobile Library
Closed
Phone: (843) 805-6909
Patron Login
menu
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Proapoptotic triterpene electrophiles (avicins) form channels in membranes: cholesterol dependence.
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
- Author(s): Li XX;Li XX; Davis B; Haridas V; Gutterman JU; Colombini M
- Source:
Biophysical journal [Biophys J] 2005 Apr; Vol. 88 (4), pp. 2577-84. Date of Electronic Publication: 2005 Jan 14.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0006-3495 (Print) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE
- Publication Information: Publication: Cambridge, MA : Cell Press
Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society. - Subject Terms: Apoptosis*; Cell Membrane/*metabolism ; Cholesterol/*chemistry ; Saponins/*chemistry; Acacia/metabolism ; Animals ; Cholesterol/metabolism ; Dose-Response Relationship, Drug ; Erythrocytes/metabolism ; Humans ; Hydrogen-Ion Concentration ; Hydrolysis ; Male ; Membrane Proteins/chemistry ; Mitochondria/metabolism ; Models, Chemical ; Models, Molecular ; Oxidation-Reduction ; Phosphatidylcholines ; Phospholipids/chemistry ; Phospholipids/metabolism ; Potassium/chemistry ; Rats ; Rats, Sprague-Dawley ; Water/chemistry
- Abstract: Avicins, a family of triterpenoid saponins from Acacia victoriae, can regulate the innate stress response in human cells. Their ability to induce apoptosis in transformed cells makes them potential anticancer agents. We report that avicins can form channels in membranes. The conductance reached a steady state after each addition, indicating a dynamic equilibrium between avicin in solution and in the membrane. The high power dependence (up to 10) of the membrane conductance on the avicin concentration indicates the formation of multimeric channels, consistent with the estimated pore radius of 1.1 nm. This radius is too small to allow protein flux across the mitochondrial outer membrane, a process known to initiate apoptosis. Channel formation is lost when avicin's amphipathic side chain is removed, implicating this as the channel-forming region. A small difference in this side chain results in strong cholesterol dependence of channel formation in avicin G that is not found in avicin D. In neutral membranes, avicin channels are nonselective, but negatively-charged lipids confer cation selectivity (5:1, K(+):Cl(-)), indicating that phospholipids form part of the permeation pathway. Avicin channels in the mitochondrial outer membrane may favor apoptosis by altering the potential across this membrane and the intermembrane space pH.
- References: Proc Natl Acad Sci U S A. 1972 Dec;69(12):3561-6. (PMID: 4509315)
Biophys J. 1999 Jan;76(1 Pt 1):281-90. (PMID: 9876141)
J Biol Chem. 1984 Aug 25;259(16):9997-10003. (PMID: 6469976)
Ann N Y Acad Sci. 1986;488:468-80. (PMID: 3555261)
Biophys J. 2000 Dec;79(6):2785-800. (PMID: 11106589)
Planta Med. 2001 Feb;67(1):43-8. (PMID: 11270721)
Proc Natl Acad Sci U S A. 2001 May 8;98(10):5821-6. (PMID: 11344312)
J Biol Chem. 2001 Jun 1;276(22):19414-9. (PMID: 11259441)
Cancer Res. 2001 Jul 15;61(14):5486-90. (PMID: 11454696)
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11551-6. (PMID: 11572997)
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11557-62. (PMID: 11572998)
Biophys J. 2002 Feb;82(2):684-92. (PMID: 11806911)
J Biol Chem. 2002 Jul 26;277(30):26796-803. (PMID: 12006562)
OMICS. 2002;6(3):235-46. (PMID: 12427275)
Cell Microbiol. 2003 Feb;5(2):99-109. (PMID: 12580946)
J Nat Prod. 2003 Jun;66(6):779-83. (PMID: 12828461)
J Clin Invest. 2004 Jan;113(1):65-73. (PMID: 14702110)
Methods Enzymol. 1987;148:465-75. (PMID: 2447469)
Adv Exp Med Biol. 1996;404:547-55. (PMID: 8957323)
Nature. 1997 Jan 23;385(6614):353-7. (PMID: 9002522)
Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11357-62. (PMID: 9326614)
Mol Cell Biochem. 1974 May 30;3(3):179-86. (PMID: 4209248) - Accession Number: 0 (Membrane Proteins)
0 (Phosphatidylcholines)
0 (Phospholipids)
0 (Saponins)
0 (avicin G)
059QF0KO0R (Water)
69279-91-0 (asolectin)
69451KN1RO (avicin D)
97C5T2UQ7J (Cholesterol)
RWP5GA015D (Potassium) - Publication Date: Date Created: 20050118 Date Completed: 20050817 Latest Revision: 20181113
- Publication Date: 20240628
- Accession Number: PMC1305354
- Accession Number: 10.1529/biophysj.104.049403
- Accession Number: 15653745
- Source:
Contact CCPL
Copyright 2022 Charleston County Public Library Powered By EBSCO Stacks 3.3.0 [350.3] | Staff Login
No Comments.