Construction of genic male sterility system by CRISPR/Cas9 editing from model legume to alfalfa.

For practical utilization in cultivated alfalfa, we designed two target sites (fused to sgRNA cassettes driven by I MtU6-6p i and I MtU6-5p i ) in the alfalfa reference genome according to the previous editing events in the diploid model (Figure 1h). Keywords: male sterile line; Medicago truncatula; Medicago sativa; gene editing; glucose-methanol-choline (GMC) oxidoreductase; NP1 EN male sterile line Medicago truncatula Medicago sativa gene editing glucose-methanol-choline (GMC) oxidoreductase NP1 613 615 3 04/11/22 20220401 NES 220401 Alfalfa ( I Medicago sativa i L.) is an important forage crop with high nutrient values. Male sterile line, Medicago truncatula, Medicago sativa, gene editing, glucose-methanol-choline (GMC) oxidoreductase, NP1. [Extracted from the article]

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