Glycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome transgenic mice.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Source:
      Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0406041 Publication Model: Print Cited Medium: Print ISSN: 0021-9967 (Print) Linking ISSN: 00219967 NLM ISO Abbreviation: J Comp Neurol Subsets: MEDLINE
    • Publication Information:
      Publication: <2003-> : Hoboken, N.J. : Wiley-Liss
      Original Publication: Philadelphia Wistar Institute of Anatomy and Biology
    • Subject Terms:
    • Abstract:
      Although glycine is a major inhibitory transmitter in the mammalian CNS, the role of glycinergic neurons in defined neuronal circuits remains ill defined. This is due in part to difficulties in identifying these cells in living slice preparations for electrophysiological recordings and visualizing their axonal projections. To facilitate the morphological and functional analysis of glycinergic neurons, we generated bacterial artificial chromosome (BAC) transgenic mice, which specifically express enhanced green fluorescent protein (EGFP) under the control of the promotor of the glycine transporter (GlyT) 2 gene, which is a reliable marker for glycinergic neurons. Neurons expressing GlyT2-EGFP were intensely fluorescent, and their dendrites and axons could be visualized in great detail. Numerous positive neurons were detected in the spinal cord, brainstem, and cerebellum. The hypothalamus, intralaminar nuclei of the thalamus, and basal forebrain also received a dense GlyT2-EGFP innervation, whereas in the olfactory bulb, striatum, neocortex, hippocampus, and amygdala positive fibers were much less abundant. No GlyT2-EGFP-positive cell bodies were seen in the forebrain. On the subcellular level, GlyT2-EGFP fluorescence was colocalized extensively with glycine immunoreactivity in somata and dendrites and with both glycine and GlyT2 immunoreactivity in axon terminals, as shown by triple staining at all levels of the neuraxis, confirming the selective expression of the transgene in glycinergic neurons. In slice preparations of the spinal cord, no difference between the functional properties of EGFP-positive and negative neurons could be detected, confirming the utility of visually identifying glycinergic neurons to investigate their functional role in electrophysiological studies.
      (2004 Wiley-Liss, Inc.)
    • Accession Number:
      0 (Amino Acid Transport Systems, Neutral)
      0 (Biomarkers)
      0 (Glycine Plasma Membrane Transport Proteins)
      0 (Luminescent Agents)
      0 (Slc6a5 protein, mouse)
      0 (enhanced green fluorescent protein)
      147336-22-9 (Green Fluorescent Proteins)
      TE7660XO1C (Glycine)
    • Publication Date:
      Date Created: 20041222 Date Completed: 20050418 Latest Revision: 20220309
    • Publication Date:
      20221213
    • Accession Number:
      10.1002/cne.20349
    • Accession Number:
      15611994