Optimal N-acetylcysteine concentration for intratympanic injection to prevent cisplatin-induced ototoxicity in guinea pigs.

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    • Subject Terms:
      ACETYLCYSTEINE; BIOLOGICAL models; AUDITORY evoked response; INJECTIONS; ANIMAL experimentation; INTRAPERITONEAL injections; COMPARATIVE studies; CISPLATIN; OTOTOXICITY; TYMPANIC membrane; DESCRIPTIVE statistics; HEARING disorders; GUINEA pigs; PHYSIOLOGIC salines; BRAIN stem
    • Abstract:
      Cisplatin is a chemotherapy drug that can induce sensorineural hearing loss. At present, no otoprotective agent is approved for use. This study investigated the optimal concentration of intratympanic N-acetylcysteine (NAC) to prevent cisplatin-induced ototoxicity in a guinea pig model. Guinea pigs (n = 64) were treated with a single intratympanic injection containing different NAC concentrations or saline (control) 3 days prior to intraperitoneal injection with cisplatin. The threshold change in the auditory brainstem response was assessed. Four weeks after intraperitoneal cisplatin injection, only the group that received 2% NAC exhibited significant otoprotection (p <.05) compared with the control. Otoprotection was observed at all the frequencies tested (1k, 2k, 4k, and 8k Hz). The 2% NAC group also exhibited significant otoprotection (p <.05) compared with the other NAC groups (at 1k, 2k, 4k, and 8k Hz). The 4% NAC group exhibited significantly reduced hearing capacity (p <.05) in the fourth week compared with controls. Intratympanic NAC administration is an efficient and safe means of preventing cisplatin-induced ototoxicity. In our animal model, the optimal intratympanic NAC concentration was 2%; concentrations of 4% loss of otoprotection. [ABSTRACT FROM AUTHOR]
    • Abstract:
      背景：顺铂是一种可诱发感音神经性听力损失的化疗药物。目前还没有任何耳保护剂获准使用。 目的：本研究调查了对于豚鼠模型, 防止顺铂诱发的耳毒性的鼓室内 N-乙酰半胱氨酸 (NAC) 的最佳浓度。 材料和方法：豚鼠（n = 64）在腹腔注射顺铂 3 天之前, 用含有不同 NAC 浓度或盐水（对照）进行单次鼓室注射治疗。评估了听觉脑干反应的阈值变化。 结果：腹腔内顺铂注射 4 周后, 与对照组相比, 只有接受 2% NAC 的组表现出显著的耳保护作用 (p <.05)。在所有测试的频率（1k、2k、4k 和 8k Hz）都观察到耳保护作用。与其他 NAC 组（1k、2k、4k 和 8k Hz）相比, 2% NAC 组也表现出显著的耳保护作用（p <.05）。 4% NAC 组与对照组相比, 第四周的听力能力显著降低 (p <.05)。 结论和意义：鼓室内 NAC 治疗是一种有效和安全的防止顺铂引起耳毒性的方法。在我们的动物模型中, 最佳鼓室内 NAC 浓度为 2%；浓度为 4% , 耳保护作用丧失。 [ABSTRACT FROM AUTHOR]