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Phone: (843) 766-6635
Wando Mount Pleasant Library
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Human placental mesenchymal stromal cells are ciliated and their ciliation is compromised in preeclampsia.
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- Author(s): Romberg, Sophia Indira1 (AUTHOR); Kreis, Nina-Naomi1 (AUTHOR); Friemel, Alexandra1 (AUTHOR); Roth, Susanne1 (AUTHOR); Souto, Alice Steglich1 (AUTHOR); Hoock, Samira Catharina1 (AUTHOR); Fischer, Kyra1 (AUTHOR); Nowak, Thorsten2 (AUTHOR); Solbach, Christine1 (AUTHOR); Louwen, Frank1 (AUTHOR); Ritter, Andreas1 (AUTHOR) ; Yuan, Juping1 (AUTHOR)
- Source:
BMC Medicine. 2/16/2022, Vol. 20 Issue 1, p1-20. 20p.- Subject Terms:
- Source:
- Additional Information
- Abstract: Background: The development of the human placenta is tightly coordinated by a multitude of placental cell types, including human chorionic villi mesenchymal stromal cells (hCV-MSCs). Defective hCV-MSCs have been reported in preeclampsia (PE), a gestational hypertensive disease characterized by maternal endothelial dysfunction and systemic inflammation. Our goal was to determine whether hCV-MSCs are ciliated and whether altered ciliation is responsible for defective hCV-MSCs in preeclamptic placentas, as the primary cilium is a hub for signal transduction, which is important for various cellular activities. Methods: In the present work, we collected placental tissues from different gestational stages and we isolated hCV-MSCs from 1st trimester, term control, and preeclamptic placentas. We studied their ciliation, functionality, and impact on trophoblastic cell lines and organoids formed from human trophoblast stem cells (hTSCs) and from the trophoblastic cell line JEG-3 with various cellular and molecular methods, including immunofluorescence staining, gene analysis, spheroid/organoid formation, motility, and cellular network formation assay. The statistical evaluation was performed using a Student's t test (two-tailed and paired or homoscedastic) or an unpaired Mann–Whitney U test (two-tailed). Results: The results show that primary cilia appeared abundantly in normal hCV-MSCs, especially in the early development of the placenta. Compared to control hCV-MSCs, the primary cilia were truncated, and there were fewer ciliated hCV-MSCs derived from preeclamptic placentas with impaired hedgehog signaling. Primary cilia are necessary for hCV-MSCs' proper signal transduction, motility, homing, and differentiation, which are impaired in preeclamptic hCV-MSCs. Moreover, hCV-MSCs derived from preeclamptic placentas are significantly less capable of promoting growth and differentiation of placental organoids, as well as cellular network formation. Conclusions: These data suggest that the primary cilium is required for the functionality of hCV-MSCs and primary cilia are impaired in hCV-MSCs from preeclamptic placentas. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of BMC Medicine is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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