PC-407 inhibited proliferation and induced apoptosis in human colon cancer SW-1116 cells.

Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 100956087 Publication Model: Print Cited Medium: Print ISSN: 1671-4083 (Print) Linking ISSN: 16714083 NLM ISO Abbreviation: Acta Pharmacol Sin Subsets: MEDLINE

Publication: 2009- : New York : Nature Publishing Group
Original Publication: Beijing, China : Science Press, c2000-

Antineoplastic Agents/*pharmacology ; Apoptosis/*drug effects ; Colonic Neoplasms/*pathology ; Cyclooxygenase Inhibitors/*pharmacology ; Naphthalenes/*pharmacology ; Sulfonamides/*pharmacology; Antineoplastic Agents/administration & dosage ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Colonic Neoplasms/ultrastructure ; Cyclooxygenase Inhibitors/administration & dosage ; DNA Fragmentation/drug effects ; Dose-Response Relationship, Drug ; Humans ; Naphthalenes/administration & dosage ; Sulfonamides/administration & dosage

Aim: To study whether PC-407 [4-[5-naphthyl-3- (trifluoromethyl)-1H-pyrazol-1-yl] benzenesulfonamide] inhibits cell viability and induces apoptosis in human colon cancer SW-1116 cells.
Methods: Inhibition of SW-1116 proliferation was measured by MTT assay. Morphological assessment of apoptosis was performed with fluorescence microscope and electron microscope. DNA fragmentation was visualized by agarose gel electrophoresis. The amount of apoptotic cells was measured by flow cytometry.
Results: PC-407 inhibited SW-1116 cell proliferation in a concentration-dependent manner after 3 d of treatment, and the IC(50) for PC-407 inhibition of cell number was 16.67+/-0.17 micromol/L. After incubation of SW-1116 cells with PC-407 20 micromol/L for 24 h, morphological changes of typical apoptosis were observed by AO/EB staining or transmission electron microscopy. Flow cytometry analysis showed that PC-407 induced apoptosis in SW-1116 cells in a time- and concentration-dependent manner. The agarose gel electrophoresis of DNA revealed a ladder pattern 48 h later.
Conclusion: PC-407 inhibited proliferation and induced apoptosis in the human colon cancer SW-1116 cell line.

0 (4-(5-naphthyl-3- (trifluoromethyl)-1H-pyrazol-1-yl)benzenesulfonamide)
0 (Antineoplastic Agents)
0 (Cyclooxygenase Inhibitors)
0 (Naphthalenes)
0 (Sulfonamides)

Date Created: 20041105 Date Completed: 20050318 Latest Revision: 20041104

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