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Neurotransmitter properties of spinal interneurons in embryonic and larval zebrafish.
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- Author(s): Higashijima S;Higashijima S; Schaefer M; Fetcho JR
- Source:
The Journal of comparative neurology [J Comp Neurol] 2004 Nov 29; Vol. 480 (1), pp. 19-37.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
- Language:
English
- Additional Information
- Source:
Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0406041 Publication Model: Print Cited Medium: Print ISSN: 0021-9967 (Print) Linking ISSN: 00219967 NLM ISO Abbreviation: J Comp Neurol Subsets: MEDLINE
- Publication Information:
Publication: <2003-> : Hoboken, N.J. : Wiley-Liss
Original Publication: Philadelphia Wistar Institute of Anatomy and Biology
- Subject Terms:
- Abstract:
Many classes of spinal interneurons in zebrafish have been described based on morphology, but their transmitter phenotypes are largely unknown. Here we combine back-filling or genetic labeling of spinal interneurons with in situ staining for markers of neurotransmitter phenotypes, including the vesicular glutamate transporter (VGLUT) genes for glutamatergic neurons, the neuronal glycine transporter (GLYT2) for glycinergic neurons, and glutamic acid decarboxylase (GAD) for GABAergic neurons. Neurons positive for VGLUT include the commissural CoPA, MCoD, UCoD, and some of the CoSA neurons. The CiD interneurons, which have ipsilateral descending axons, were also VGLUT-positive, as were the ventrally located VeMe interneurons, whose descending axonal trajectory has not been clearly revealed. Cells positive for GLYT2 include the commissural CoLAs as well as some of the CoBL and CoSA neurons. The CiA cells were the only GLYT2-positive cells with an ipsilateral axon. Cells staining for GAD included, most notably, the dorsal longitudinal ascending (DoLA) and KA interneurons. Our approach allowed us to define the likely transmitter phenotypes of most of the known classes of spinal interneurons. These data provide a foundation for understanding the functional organization of the spinal networks in zebrafish.
- Grant Information:
R01 NS026539 United States NS NINDS NIH HHS; R01 NS026539-25 United States NS NINDS NIH HHS; NS 26539 United States NS NINDS NIH HHS
- Accession Number:
0 (Amino Acid Transport Systems, Neutral)
0 (Biomarkers)
0 (Glycine Plasma Membrane Transport Proteins)
0 (Isoenzymes)
0 (Membrane Transport Proteins)
0 (Neurotransmitter Agents)
0 (RNA, Messenger)
0 (Vesicular Glutamate Transport Protein 1)
147336-22-9 (Green Fluorescent Proteins)
3KX376GY7L (Glutamic Acid)
56-12-2 (gamma-Aminobutyric Acid)
EC 4.1.1.15 (Glutamate Decarboxylase)
EC 4.1.1.15 (glutamate decarboxylase 1)
TE7660XO1C (Glycine)
- Publication Date:
Date Created: 20041030 Date Completed: 20050512 Latest Revision: 20161122
- Publication Date:
20221213
- Accession Number:
10.1002/cne.20279
- Accession Number:
15515025
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