A Novel In Vivo Functional Screening Method for the Candidate Polyphosphate Accumulating Organisms Isolation.

A novel candidate PAOs screening method based on the physiological role of polyphosphate as phosphorus supply under the phosphorus starvation conditions was developed. By successive inoculation between phosphorus rich and phosphorus starvation media, 20 strains of Acinetobacter, Arthrobacter, Comamonas, Trichosporon, Cryptococcus and Cyberlindnera were isolated and revealed positive for metachromatic granules staining. Fractional extraction demonstrated all the strains could accumulate polyphosphate. Among of them, Cryptococcus and Cyberlindnera were first isolated as candidate PAOs. Acinetobacter sp. SWA4-16 strain mutated by N-methyl-N'-nitro-N-nitrosoguanidine treatment significantly improved its phosphorus removal rate, phosphorus content and biomass. The mutant strain could remove more than 28 mg/L phosphorus by overnight culture and accumulate 2.4-fold more phosphorus on dry weight (to 8.92%) accompanied by an increase of biomass for 1.2-fold. This study combing a new method for screening microorganisms capable of accumulating phosphorus in the form of polyphosphates and the mutant method for constitute expression of phosphate-specific transport, is promising to isolate the high performance candidate PAOs efficient for wastewater polyphosphate removal. [ABSTRACT FROM AUTHOR]

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