An experimental model of allergic asthma in cats sensitized to house dust mite or bermuda grass allergen.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: S. Karger Country of Publication: Switzerland NLM ID: 9211652 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1018-2438 (Print) Linking ISSN: 10182438 NLM ISO Abbreviation: Int Arch Allergy Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel ; New York : S. Karger, 1992-
    • Subject Terms:
      Disease Models, Animal*; Asthma/*immunology ; Asthma/*physiopathology ; Cynodon/*immunology ; Hypersensitivity/*immunology ; Pyroglyphidae/*immunology; Animals ; Asthma/complications ; Bronchial Hyperreactivity/etiology ; Bronchial Hyperreactivity/immunology ; Bronchial Provocation Tests ; Bronchoalveolar Lavage Fluid/immunology ; Cats ; Cytokines/biosynthesis ; Enzyme-Linked Immunosorbent Assay ; Eosinophilia/etiology ; Hypersensitivity, Immediate/immunology ; Hypersensitivity, Immediate/physiopathology ; Immunoglobulin A/analysis ; Immunoglobulin E/blood ; Immunoglobulin G/analysis ; Lung/immunology ; Lung/pathology ; Lymphocyte Activation/immunology ; Lymphocytes/immunology
    • Abstract:
      Background: Animal models are used to mimic human asthma, however, not all models replicate the major characteristics of the human disease. Spontaneous development of asthma with hallmark features similar to humans has been documented to occur with relative frequency in only one animal species, the cat. We hypothesized that we could develop an experimental model of feline asthma using clinically relevant aeroallergens identified from cases of naturally developing feline asthma, and characterize immunologic, physiologic, and pathologic changes over 1 year.
      Methods: House dust mite (HDMA) and Bermuda grass (BGA) allergen were selected by screening 10 privately owned pet cats with spontaneous asthma using a serum allergen-specific IgE ELISA. Parenteral sensitization and aerosol challenges were used to replicate the naturally developing disease in research cats. The asthmatic phenotype was characterized using intradermal skin testing, serum allergen-specific IgE ELISA, serum and bronchoalveolar lavage fluid (BALF) IgG and IgA ELISAs, airway hyperresponsiveness testing, BALF cytology, cytokine profiles using TaqMan PCR, and histopathologic evaluation.
      Results: Sensitization with HDMA or BGA in cats led to allergen-specific IgE production, allergen-specific serum and BALF IgG and IgA production, airway hyperreactivity, airway eosinophilia, an acute T helper 2 cytokine profile in peripheral blood mononuclear cells and BALF cells, and histologic evidence of airway remodeling.
      Conclusions: Using clinically relevant aeroallergens to sensitize and challenge the cat provides an additional animal model to study the immunopathophysiologic mechanisms of allergic asthma. Chronic exposure to allergen in the cat leads to a variety of immunologic, physiologic, and pathologic changes that mimic the features seen in human asthma.
    • Grant Information:
      ES07055 United States ES NIEHS NIH HHS
    • Accession Number:
      0 (Cytokines)
      0 (Immunoglobulin A)
      0 (Immunoglobulin G)
      37341-29-0 (Immunoglobulin E)
    • Publication Date:
      Date Created: 20040904 Date Completed: 20041119 Latest Revision: 20071114
    • Publication Date:
      20221213
    • Accession Number:
      10.1159/000080654
    • Accession Number:
      15345910