Ethyl ferulate, a lipophilic polyphenol, induces HO-1 and protects rat neurons against oxidative stress.

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  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 100888899 Publication Model: Print Cited Medium: Print ISSN: 1523-0864 (Print) Linking ISSN: 15230864 NLM ISO Abbreviation: Antioxid Redox Signal Subsets: MEDLINE
    • Publication Information:
      Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., 1999-
    • Subject Terms:
      Enzyme Induction* ; Oxidative Stress*; Caffeic Acids/*pharmacology ; Heme Oxygenase (Decyclizing)/*metabolism ; Neurons/*drug effects ; Neuroprotective Agents/*pharmacology; Animals ; Antioxidants/chemistry ; Antioxidants/pharmacology ; Astrocytes/cytology ; Astrocytes/drug effects ; Astrocytes/metabolism ; Caffeic Acids/chemistry ; Caffeic Acids/metabolism ; Cell Survival ; Heme Oxygenase (Decyclizing)/genetics ; Heme Oxygenase-1 ; Neurons/cytology ; Neurons/metabolism ; Neuroprotective Agents/metabolism ; RNA, Messenger/metabolism ; Rats ; Resveratrol ; Stilbenes/chemistry ; Stilbenes/pharmacology
    • Abstract:
      In the CNS, the heme oxygenase (HO) system has been reported to be active and to operate as a fundamental defensive mechanism for neurons exposed to an oxidant challenge. We have recently shown that both curcumin and caffeic acid phenethyl ester, two phenolic natural compounds, potently induce HO-1 expression and activity in rat astrocytes. We have extended our previous findings examining the effects of two other plant-derived phenolic compounds, with analogous chemical structures, in rat astrocytes and neurons. Ethyl ferulate (ethyl 4-hydroxy-3-methoxycinnamate) (EFE), the naturally occurring ester of ferulic acid, was able to induce HO-1 protein expression. Maximal expression of HO-1 mRNA and protein and a significant increase in HO activity were detected after 6 h of incubation with 15 microM EFE in astrocytes and 5 microM EFE in neurons. Higher concentrations of EFE (50 microM) caused a substantial cytotoxic effect with no change in HO-1 protein expression and activity. Exposure of astrocytes to resveratrol, a phytoalexin derived from grapes, resulted in an increase of HO-1 mRNA, but it was not able to induce HO-1 protein expression and activity. Interestingly, preincubation (12 h) of neurons with EFE resulted in an enhanced cellular resistance to glucose oxidase-mediated oxidative damage; this cytoprotective effect was considerably attenuated by zinc protoporphyrin IX, an inhibitor of HO activity. This study identifies a novel natural compound that could be used for therapeutic purposes as a potent inducer of HO-1 for the protection of brain cells against oxidative and neurodegenerative conditions.
    • Accession Number:
      0 (Antioxidants)
      0 (Caffeic Acids)
      0 (Neuroprotective Agents)
      0 (RNA, Messenger)
      0 (Stilbenes)
      5B8915UELW (ethyl ferulate)
      EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
      EC 1.14.14.18 (Heme Oxygenase-1)
      EC 1.14.14.18 (heme oxygenase-2)
      Q369O8926L (Resveratrol)
    • Publication Date:
      Date Created: 20040904 Date Completed: 20050816 Latest Revision: 20181130
    • Publication Date:
      20231215
    • Accession Number:
      10.1089/ars.2004.6.811
    • Accession Number:
      15345140