Gelatin particle indirect agglutination and enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis using Strongyloides venezuelensis antigen.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 7506129 Publication Model: Print Cited Medium: Print ISSN: 0035-9203 (Print) Linking ISSN: 00359203 NLM ISO Abbreviation: Trans R Soc Trop Med Hyg Subsets: MEDLINE
    • Publication Information:
      Publication: 2013- : Oxford : Oxford University Press
      Original Publication: 1920- : London : Royal Society of Tropical Medicine and Hygiene
    • Subject Terms:
      Agglutination Tests/*methods ; Enzyme-Linked Immunosorbent Assay/*methods ; Strongyloidiasis/*diagnosis; Adolescent ; Adult ; Aged ; Agglutination Tests/standards ; Analysis of Variance ; Animals ; Antigens, Helminth/analysis ; Child ; Child, Preschool ; Enzyme-Linked Immunosorbent Assay/standards ; HTLV-I Infections/complications ; Humans ; Middle Aged ; Sensitivity and Specificity ; Strongyloides/immunology ; Strongyloides/isolation & purification
    • Abstract:
      Routine microscopical examination of stool specimens for diagnosis of strongyloidiasis is insensitive and serological methods using Strongyloides stercoralis antigen are at present not available for field studies. We evaluated 2 techniques, enzyme-linked immunosorbent assay (ELISA) and gelatin particle indirect agglutination (GPIA), using an antigen obtained from the rodent parasite, S. venezuelensis. Fifty-four Peruvian patients with different clinical forms of strongyloidiasis were studied: 12 asymptomatic, 31 symptomatic, and 11 hyperinfection cases. Our results demonstrate that both ELISA and GPIA using S. venezuelensis antigen are useful for diagnosis of strongyloidiasis, with sensitivities of 74.1% and 98.2%, respectively and a specificity of 100% for both techniques. We found that GPIA is a highly sensitive test for patients with suspected chronic infection and/or hyperinfection. In the hyperinfection cases, significantly lower concentrations of specific immunoglobulin antibodies and eosinophils (P < 0.001) were found compared with the asymptomatic and symptomatic cases.
    • Accession Number:
      0 (Antigens, Helminth)
    • Publication Date:
      Date Created: 20040817 Date Completed: 20041108 Latest Revision: 20191210
    • Publication Date:
      20250114
    • Accession Number:
      10.1016/s0035-9203(03)80017-2
    • Accession Number:
      15307419