[In vitro culture and biological characteristics of cranial neural crest stem cell].

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  • Author(s): Tian WD;Tian WD; Jiang HB; Liu L; Tang W; Li XD
  • Source:
    Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology [Hua Xi Kou Qiang Yi Xue Za Zhi] 2004 Jun; Vol. 22 (3), pp. 229-31.
  • Publication Type:
    English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Sichuan yi xue yuan Country of Publication: China NLM ID: 9422648 Publication Model: Print Cited Medium: Print ISSN: 1000-1182 (Print) Linking ISSN: 10001182 NLM ISO Abbreviation: Hua Xi Kou Qiang Yi Xue Za Zhi Subsets: MEDLINE
    • Publication Information:
      Publication: Chengdu : Sichuan yi xue yuan
      Original Publication: Chengdu : Sichuan yi xue yuan, 1983-
    • Subject Terms:
      Cranial Nerves/*cytology ; Neural Crest/*cytology ; Stem Cells/*cytology; Animals ; Cell Differentiation ; Female ; In Vitro Techniques ; Male ; Mice
    • Abstract:
      Objective: According to development biology, cranial neural crest stem cell (CNCSC) can differentiate into precursor cells of tooth, jaw and peripheral nerve system, but in vitro study is less reported. In the present study, CNCSC were dissociated and cultured in vitro, and the biological characteristics of CNCSC was investigated.
      Methods: Cranial neural tubes, dissected from mouse E9d, were explanted onto fibronectin-coated dishes. CNCSCs emigrated from the explanted neural tubes, cultured in a serum-free medium containing modified DMEM/F12. Biological characteristics of CNCSC were detected by morphology, nuclear labeled with BurdU and immunocytochemistry.
      Results: Fibroblast-like CNCSCs demonstrated the characteristics of stem cell, such as clonality, self-renewal and multipotentiality. The result of immunocytochemical stain showed that CNCSC expressed HNK-1 antigen.
      Conclusion: CNCSC were cultured successfully, providing a experimental basis for study on tooth/jaw-like differentiation of CNCSC in vitro, especially providing a cell source for investigating tooth/jaw regeneration.
    • Publication Date:
      Date Created: 20040806 Date Completed: 20070206 Latest Revision: 20141120
    • Publication Date:
      20231215
    • Accession Number:
      15293472