[Effect of previous culture conditions and the presence of the rpoS gene on the survival of Salmonella typhimurium in sea water exposed to sunlight].

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  • Additional Information
    • Transliterated Title:
      Influence des conditions de culture préalables et de la présence du gène rpoS pour la survie de Salmonella typhimurium en eau de mer exposée à la lumière solaire.
    • Source:
      Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 0372707 Publication Model: Print Cited Medium: Print ISSN: 0008-4166 (Print) Linking ISSN: 00084166 NLM ISO Abbreviation: Can J Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : Ottawa, ON : Canadian Science Publishing
      Original Publication: Ottawa, Canada : National Research Council, [1954-
    • Subject Terms:
      Sunlight*; Bacterial Proteins/*genetics ; Salmonella typhimurium/*genetics ; Salmonella typhimurium/*radiation effects ; Seawater/*microbiology ; Sigma Factor/*genetics; Adaptation, Physiological ; Amino Acids/metabolism ; Bacterial Proteins/physiology ; Colony Count, Microbial ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Hydrogen Peroxide/metabolism ; Mutation ; Osmolar Concentration ; Salmonella typhimurium/growth & development ; Sigma Factor/physiology ; Water Microbiology
    • Abstract:
      The effect of sunlight exposure on Salmonella typhimurium isogenic strains harboring an rpoS gene functional (rpoS+) or not functional (rpoS-) was investigated in microcosms of sterile sea water at 20 degrees C. The two strains rapidly lost their ability to produce colonies on solid culture media. The detrimental action of sunlight was more important when the salinity of sea water increased. The survival of stationary phase cells was influenced by RpoS. Bacteria grown in media with high salinity or osmolarity and transferred to sea water in stationary phase were more resistant to irradiation than those grown in media with low salinity. Prior growth under oxidative (0.2 mmol/L of H2O2) or amino acid starved (minimal medium) conditions did not modify the survival of either strain when they were exposed to sunlight. Bacteria were more resistant when cells were incubated in sea water in the dark prior to being exposed to sunlight. The resistance to sunlight irradiation was also greater in clones of both strains isolated from microcosms exposed to sunlight for 90 min, then further inoculated into sea water and reexposed to sunlight.
    • Accession Number:
      0 (Amino Acids)
      0 (Bacterial Proteins)
      0 (Sigma Factor)
      0 (sigma factor KatF protein, Bacteria)
      BBX060AN9V (Hydrogen Peroxide)
    • Publication Date:
      Date Created: 20040624 Date Completed: 20041014 Latest Revision: 20131121
    • Publication Date:
      20231215
    • Accession Number:
      10.1139/w04-011
    • Accession Number:
      15213742