Detection of Shigella by a PCR assay targeting the ipaH gene suggests increased prevalence of shigellosis in Nha Trang, Vietnam.

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  • Additional Information
    • Source:
      Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print Cited Medium: Print ISSN: 0095-1137 (Print) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Society for Microbiology.
    • Subject Terms:
      Dysentery, Bacillary/*epidemiology ; Dysentery, Bacillary/*microbiology ; Polymerase Chain Reaction/*methods ; Shigella/*genetics ; Shigella/*isolation & purification; Adolescent ; Adult ; Antigens, Bacterial/genetics ; Bacterial Proteins/genetics ; Base Sequence ; Child ; Child, Preschool ; DNA, Bacterial/genetics ; Dysentery, Bacillary/diagnosis ; Female ; Genes, Viral ; Humans ; Infant ; Male ; Middle Aged ; Vietnam/epidemiology
    • Abstract:
      Shigella spp. are exquisitely fastidious gram-negative organisms which frequently escape detection by traditional culture methods. To get a more complete understanding of the disease burden caused by Shigella in Nha Trang, Vietnam, real-time PCR was used to detect Shigella DNA. Randomly selected rectal swab specimens from 60 Shigella culture-positive patients and 500 Shigella culture-negative patients detected by population-based surveillance of patients seeking care for diarrhea were processed by real-time PCR. The target of the primer pair is the invasion plasmid antigen H gene sequence (ipaH), carried by all four Shigella species and enteroinvasive Escherichia coli. Shigella spp. could be isolated from the rectal swabs of 547 of 19,206 (3%) patients with diarrhea. IpaH was detected in 55 of 60 (93%) Shigella culture-positive specimens, whereas it was detected in 87 of 245 (36%) culture-negative patients free of dysentery (P < 0.001). The number of PCR cycles required to detect a PCR product was highest for culture-negative, nonbloody diarrheal specimens (mean number of cycles to detection, 36.6) and was lowest for children with culture-positive, bloody diarrheal specimens (mean number of cycles, 25.3) (P < 0.001). The data from real-time PCR amplification indicate that the culture-proven prevalence of Shigella among patients with diarrhea may underestimate the prevalence of Shigella infections. The clinical presentation of shigellosis may be directly related to the bacterial load.
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    • Accession Number:
      0 (Antigens, Bacterial)
      0 (Bacterial Proteins)
      0 (DNA, Bacterial)
      0 (ipaH protein, Shigella flexneri)
    • Publication Date:
      Date Created: 20040508 Date Completed: 20040902 Latest Revision: 20220410
    • Publication Date:
      20250114
    • Accession Number:
      PMC404673
    • Accession Number:
      10.1128/JCM.42.5.2031-2035.2004
    • Accession Number:
      15131166