Alteraciones histopatológicas en el hígado del sapo Peltophryne empusa (Anura: Bufonidae) del Occidente de Cuba: ¿señal de alarma?

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    • Alternate Title:
      Histopathological alterations in the liver of the Peltophryne empusa toad (Anura: Bufonidae) from Western Cuba: alarm signal?
    • Subject Terms:
      AMPHIBIAN declines; LIVER histology; POLLUTION; TRANSMISSION electron microscopy; HISTOLOGY; EOSIN; GLUTARALDEHYDE
    • Subject Terms:
      CUBA
    • Abstract:
      Durante las últimas dos décadas ha habido una disminución alarmante en las poblaciones de anfibios a nivel global. Entre las causas de este fenómeno se encuentra la contaminación ambiental por factores químicos los cuales pueden tener efectos muy nocivos en hígado y riñones. Los objetivos del presente trabajo fueron describir la histología del hígado de Peltophryne empusa y sugerir una posible explicación para la abundante pigmentación detectada en su parénquima. Se recolectaron dos hembras y dos machos en una zona agrícola de la provincia Artemisa, Cuba. Los ejemplares se disectaron; fragmentos de hígado se fijaron en paraformaldehido al 4% y fueron sometidos a la técnica clásica de inclusión en parafina. Los cortes se tiñeron con hematoxilina y eosina, tricrómica de Mallory y tricrómica de Gomory. Otras porciones de hígado se fijaron en glutaraldehído al 2,5% y se procesaron para microscopía electrónica de transmisión. Los resultados evidenciaron que la histología del hígado era similar a la de otros anfibios; sin embargo, numerosos cuerpos pigmentarios observados fueron centros de melanomacrófagos derivados de células de Kupffer, cuya presencia y abundancia se discute en el presente trabajo. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Over the past two decades there has been an alarming decline in amphibian populations globally. Among the causes of this phenomenon is environmental pollution by chemical factors which can have very harmful effects on the liver and kidneys. The objectives of the present work was to describe the histology of the Peltophryne empusa liver, suggesting a possible explanation for the abundant pigmentation detected in its parenchyma. Two females and two males were collected in an agricultural area of Artemisa province, Cuba. The specimens were dissected; liver fragments were fixed in 4% paraformaldehyde and were subjected to the classic paraffin embedding technique. The sections were stained with hematoxylin and eosin, Mallory's trichrome and Gomory's trichrome. Other liver portions were fixed in 2.5% glutaraldehyde and processed for transmission electron microscopy. The results showed that the liver histology was similar to that of other amphibians; however, numerous pigment bodies observed were Kupffer cell-derived melanomacrophage centers, the presence and abundance of which is discussed in the present work. [ABSTRACT FROM AUTHOR]
    • Abstract:
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