Where Has My Efficiency Gone? Impacts of Extracolumn Peak Broadening on Performance, Part II: Sample Injection.

Dispersion (broadening, or spreading) of analyte zones (peaks) outside of chromatography columns can seriously erode the resolution provided by good columns. In this installment, we focus on the contribution of the sample injection step to the total level of extracolumn dispersion in a liquid chromatography (LC) system. This contribution has both a physical component (that is impacted by injector design and configuration and method variables) and a chemical component (that depends on the difference between the mobile phase and the sample solvent compositions). [ABSTRACT FROM AUTHOR]

Copyright of LC-GC North America is the property of MJH Life Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)