SIMPLE AND RAPID CULTIVATION OF TRYPANOSOMA DANILEWSKYI STRAIN FCC-1 IN MONOPHASIC LIQUID CULTURE MEDIUM

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  • Author(s): By:M. S. Ahmed K. S. Ahmed W. A. Khan H. Ali F. Ollev
  • Source:
    Journal of Animal and Plant Sciences, The (Lahore, Pakistan), February 28, 2014 News, 7pp
  • Additional Information
    • Abstract:
      ABSTRACT Trypanosoma danilewskyi strain F Cc-1 was isolated from the blood of a laboratory infected juvenile common carp. Trypanosomes (blood stream forms) were first adapted to culture conditions in Tobie's medium. Ten sterilized glass culture tubes each containing 2 ml of Tobie's medium solidified in slants and covered with 1 ml Locke-Krebs overlay were inoculated with 0.1 ml of infected blood containing 1x 105 live trypanosomes and incubated at 20 C...