IL‐17A expressed on neutrophil extracellular traps promotes mesenchymal stem cell differentiation toward bone‐forming cells in ankylosing spondylitis.

Ankylosing spondylitis (AS) is an inflammatory disease characterized by excessive bone formation. We investigated the presence of neutrophil extracellular traps (NETs) in AS and how they are involved in the osteogenic capacity of bone marrow mesenchymal stem cells (MSCs) through interleukin‐17A (IL‐17A). Peripheral neutrophils and sera were obtained from patients with active AS and healthy controls. NET formation and neutrophil/NET‐associated proteins were studied using immunofluorescence, immunoblotting, qPCR, and ELISA. In vitro co‐culture systems of AS NET structures and MSCs isolated from controls were deployed to examine the role of NETs in the differentiation of MSCs toward osteogenic cells. Analysis was performed using specific staining and qPCR. Neutrophils from patients with AS were characterized by enhanced formation of NETs carrying bioactive IL‐17A and IL‐1β. IL‐17A‐enriched AS NETs mediated the differentiation of MSCs toward bone‐forming cells. The neutrophil expression of IL‐17A was positively regulated by IL‐1β. Blocking IL‐1β signaling on neutrophils with anakinra or dismantling NETs using DNase‐I disrupted osteogenesis driven by IL‐17A‐bearing NETs. These findings propose a novel role of neutrophils in AS‐related inflammation, linking IL‐17A‐decorated NETs with the differentiation of MSCs toward bone‐forming cells. Moreover, IL‐1β triggers the expression of IL‐17A on NETs offering an additional therapeutic target in AS. [ABSTRACT FROM AUTHOR]

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