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John L. Dart Library
Closed for Maintenance
Phone: (843) 722-7550
West Ashley Library
9 a.m. - 5 p.m.
Phone: (843) 766-6635
Folly Beach Library
9 a.m. - 2 p.m.
*open the 2nd and 4th Saturday
*open the 2nd and 4th Saturday
Phone: (843) 588-2001
Edgar Allan Poe/Sullivan's Island Library
Closed for renovations
Phone: (843) 883-3914
Wando Mount Pleasant Library
9 a.m. - 5 p.m.
Phone: (843) 805-6888
Village Library
9 a.m. - 1 p.m.
Phone: (843) 884-9741
St. Paul's/Hollywood Library
9 a.m. - 5 p.m.
Phone: (843) 889-3300
Otranto Road Library
9 a.m. - 5 p.m.
Phone: (843) 572-4094
Mt. Pleasant Library
9 a.m. – 5 p.m.
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McClellanville Library
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Keith Summey North Charleston Library
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9 a.m. - 5 p.m.
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Phone: (843) 805-6930
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Gene Set Enrichment Analysis of Selenium-Deficient and High-Selenium Rat Liver Transcript Expression and Comparison With Turkey Liver Expression.
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- Author(s): Sunde, Roger A
- Source:
Journal of Nutrition; Apr2021, Vol. 151 Issue 4, p772-784, 13p- Subject Terms:
GENES; WILD turkey; RATS; LIVER; DISEASE progression; BIOCHEMISTRY; PROTEOMICS; RNA metabolism; PROTEIN metabolism; PROTEINS; RESEARCH; POULTRY; ANIMAL experimentation; RESEARCH methodology; RNA; MEDICAL cooperation; EVALUATION research; GENE expression; DIETARY supplements; COMPARATIVE studies; IMMUNITY; OXIDOREDUCTASES; SELENIUM - Source:
- Additional Information
- Abstract:
Background: Better biomarkers of selenium (Se) status and a better understanding of toxic Se biochemistry are needed to set safe dietary upper limits. In previous studies, differential expression (DE) of individual liver transcripts in rats and turkeys failed to identify a single transcript that was consistently and significantly (q < 0.05) altered by high Se.Objectives: To evaluate the effect of Se status on rat liver transcript expression data at the level of gene sets, and to compare transcript expression in rats with that in turkeys to identify common regulated transcripts.Methods: Gene set enrichment analysis (GSEA) was conducted on liver from weanling rats fed an Se-deficient basal diet (0.005 μg Se/g) supplemented with 0, 0.24 (Se-adequate), 2, or 5 μg Se/g diet as selenite for 28 d. In addition, transcript expression was compared with liver expression in turkeys fed 0, 0.4, 2, or 5 μg Se/g diet as selenite.Results: Se deficiency significantly downregulated the rat selenoprotein gene set but also upregulated gene sets for a variety of pathways, processes, and disease states. GSEA of 2 compared with 0.24 μg Se/g found no significantly up- or downregulated gene sets, showing that 2 μg Se/g is not particularly toxic to the rat. GSEA analysis of 5 compared with 0.24 μg Se/g transcripts, however, found 27 significantly upregulated gene sets for a wide variety of conditions. Cross-species GSEA comparison of transcript expression, however, identified no common gene sets significantly and consistently regulated by high Se in rats and turkeys. In addition, comparison of individual marginally significant (unadjusted P < 0.05) DE transcripts between rats and turkeys also failed to find common transcripts.Conclusions: The dramatic increase in significant liver transcript DE and GSEA gene sets in rats fed 5 compared with 2 μg Se/g clearly appears to be a biomarker for Se toxicity, albeit not Se-specific. These analyses, however, failed to identify specific transcripts or pathways, biological states, or processes that were directly linked with high Se status, strongly indicating that adaptation to high Se lies outside transcriptional regulation. [ABSTRACT FROM AUTHOR] - Abstract: Copyright of Journal of Nutrition is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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