羊肚菌单孢菌株的性亲和与营养体亲和特性. (Chinese)

Sexual and Vegetative Compatibility of Single Ascospore Isolations in the Genus Morchella. (English)

Single ascospore isolations were obtained from ascocarps of Y AASMYPL6 and Zhao00Ol of Morchella importuna, Y AASMJN5 of M. sextelata and Mes-19 Y AASM43 by capillary-isolation. These single ascospore isolat ions were analyzed for their mating type genes. The results showed that the four singleascospore populations contained either MAT] 1 1 or MAT] 2 1 with a ratio of about 1 : 1. A small number of single ascospore isolations of Zhao000l, Y AASMJN5 and Y AASM43 had both MATl 1 1 and MATl 2 1, and homokaryons with only one mating type gene were obtained from these isolations by mycelium-tip subculture, suggesting that some ascospores in Morchella were heterokaryotic. Paired-cultivation for singleascospore isolation populations of Y AASMYPL6, Zhao00Ol and Y AASM43 showed that there was very few vegetative compatibility in MATl 1 X MATl 1, MATl 2 X MATl 2 or MATl 1 X MATl 2. Whether the phenomenon that two strains are sexual compatible but at the same time vegetative incompatible exists in crossbreeding of basidiomycete fungi is st ill awaiting more attentions. [ABSTRACT FROM AUTHOR]

以人工栽培梯校羊肚菌(Morchella importuna)千囊果 YAASMYPL6 和六妹羊肚菌(M. sextelata)子 囊果YAASMJN5.野生梯校羊肚菌子果 Zhiyi001 和黃色羊肚菌(Mes-19)子囊果YAASM43为材料,不用 毛细管单孢分离法分离其子囊抱子获得单孢菌株.并通过基于交配型基因的分子标记方法鉴定单在菌株的交 配型,结果显示14个样本群体中其有交配型基因 MAT1-1-1和 MAT 12-1的单孢菌株比例为1:1.3个子囊果 的单胞菌株群体中检测到同时具有MATI-1-1和MAT1-21的单型菌株,且这样的营体经过多次失端菌丝传 代培养后可获得仅有一种交配型基因的同核体商株,证明单抱菌株群体中存在一定比率的异核体的株,在 YAASMYPL6.Zhao0001和YAASM43 3个样本群体内选择具有相同和不同交配型基因的 单孢菌株进行对 時培养。发现对蜂组合中表现营养体和的组合数量极少,性亲和但因营养体不亲和面表现出菌丝指抗的现 象。在已知食用担子菌的杂交有种中是否存在尚未被关注. [ABSTRACT FROM AUTHOR]

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