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Use of protein biotinylation in vivo for chromatin immunoprecipitation.
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- Additional Information
- Source:
Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print Cited Medium: Print ISSN: 0003-2697 (Print) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE
- Publication Information:
Publication: <2000- > : San Diego, CA : Elsevier
Original Publication: Orlando Fl : Academic Press
- Subject Terms:
- Abstract:
We describe a system designed to express biotinylated proteins in mammalian cells in vivo and its application to the study of protein-DNA interactions in vivo by chromatin immunoprecipitation (ChIP). The system is based on coexpression of the target protein fused to a short biotin acceptor domain together with the biotinylating enzyme BirA from Escherichia coli. The superior strength of the biotin-avidin interaction allows one to employ more stringent washing conditions in the ChIP protocol, resulting in a better signal/noise ratio.
- Accession Number:
0 (Cell Cycle Proteins)
0 (Chromatin)
0 (Chromosomal Proteins, Non-Histone)
0 (DNA-Binding Proteins)
0 (Escherichia coli Proteins)
0 (Immunosorbents)
0 (Luminescent Proteins)
0 (Nuclear Proteins)
0 (Recombinant Fusion Proteins)
0 (Repressor Proteins)
0 (Transcription Factor DP1)
0 (Transcription Factors)
107283-02-3 (Chromobox Protein Homolog 5)
147336-22-9 (Green Fluorescent Proteins)
EC 6.3.- (Carbon-Nitrogen Ligases)
EC 6.3.4.15 (birA protein, E coli)
- Publication Date:
Date Created: 20040113 Date Completed: 20040817 Latest Revision: 20211203
- Publication Date:
20240829
- Accession Number:
10.1016/j.ab.2003.10.015
- Accession Number:
14715286
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