Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: monitoring gene expression using cDNA microarrays combined with real-time RT-PCR.

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  • Author(s): Pfaffl MW;Pfaffl MW; Gerstmayer B; Bosio A; Windisch W
  • Source:
    The Journal of nutritional biochemistry [J Nutr Biochem] 2003 Dec; Vol. 14 (12), pp. 691-702.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: United States NLM ID: 9010081 Publication Model: Print Cited Medium: Print ISSN: 0955-2863 (Print) Linking ISSN: 09552863 NLM ISO Abbreviation: J Nutr Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: <1996->: New York, NY : Elsevier Science
      Original Publication: Stoneham, MA, USA : Butterworths, c1990-
    • Subject Terms:
      Gene Expression* ; Oligonucleotide Array Sequence Analysis* ; Reverse Transcriptase Polymerase Chain Reaction*; Jejunum/*chemistry ; Liver/*chemistry ; RNA, Messenger/*analysis ; Zinc/*deficiency; Animals ; B-Lymphocytes ; Female ; Metallothionein/genetics ; RNA/analysis ; Rats
    • Abstract:
      In the study presented here, the effect of zinc deficiency on mRNA expression levels in liver and jejunum of adult rats was analyzed. Feed intake was restricted to 8 g/day. The semi-synthetic diet was fortified with pure phytate and contained either 2 microg Zn/g (Zn deficiency, n = 6) or 58 microg Zn/g (control, n = 7). After 29 days of Zn depletion feeding, entire jejunum and liver were retrieved and total RNA was extracted. Tissue specific expression pattern were screened and quantified by microarray analysis and verified individually via real-time RT-PCR. A relative quantification was performed with the newly developed Relative Expression Software Tool Copyright on numerous candidate genes which showed a differential expression. This study provides the first comparative view of gene expression regulation and fully quantitative expression analysis of 35 candidate genes in a non-growing Zn deficient adult rat model. The expression results indicate the existence of individual expression pattern in liver and jejunum and their tissue specific regulation under Zn deficiency. In addition, in jejunum a number of B-cell related genes could be demonstrated to be suppressed at Zn deficiency. In liver, metallothionein subtype 1 and 2 (MT-1 and MT-2) genes could be shown to be dramatically repressed and therefore represent putative markers for Zn deficiency. Expression results imply that some genes are expressed constitutively, whereas others are highly regulated in tissues responsible for Zn homeostasis.
    • Accession Number:
      0 (RNA, Messenger)
      63231-63-0 (RNA)
      9038-94-2 (Metallothionein)
      J41CSQ7QDS (Zinc)
    • Publication Date:
      Date Created: 20031224 Date Completed: 20040722 Latest Revision: 20191108
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.jnutbio.2003.08.007
    • Accession Number:
      14690761