Characterization of osteocalcin (BGP) and matrix Gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates.

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  • Additional Information
    • Source:
      Publisher: Springer Verlag Country of Publication: United States NLM ID: 7905481 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0171-967X (Print) Linking ISSN: 0171967X NLM ISO Abbreviation: Calcif Tissue Int Subsets: MEDLINE
    • Publication Information:
      Publication: New York Ny : Springer Verlag
      Original Publication: Berlin, New York, Springer International.
    • Subject Terms:
      Extracellular Matrix Proteins*; Antibody Specificity/*immunology ; Bone and Bones/*immunology ; Calcium-Binding Proteins/*immunology ; Fishes/*immunology ; Osteocalcin/*immunology ; Xenopus/*immunology; Amino Acid Sequence ; Animals ; Bone and Bones/metabolism ; Calcium-Binding Proteins/metabolism ; Electrophoresis, Polyacrylamide Gel ; Fishes/metabolism ; Molecular Sequence Data ; Osteocalcin/metabolism ; Species Specificity ; Xenopus/metabolism ; Matrix Gla Protein
    • Abstract:
      In fish species the basic mechanisms of bone development and bone remodeling are not fully understood. The classification of bone tissue in teleosts as cellular or acellular and the presence of transitional states between bone and cartilage and the finding of different types of cartilage in teleosts not previously recognized in higher vertebrates emphasizes the need for a study on the accumulation of the Gla-containing proteins MGP and BGP at the cellular level. In the present study, polyclonal antibodies developed against BGP and MGP from A. regius (a local marine teleost fish) and against MGP from G. galeus (a Pacific Ocean shark), were tested by Western blot for their specificity against BGP and MGP from several other species of teleost fish and shark. For this purpose we extracted and purified both proteins from various marine and freshwater teleosts, identified them by N-terminal amino acid sequence analysis and confirmed the presence of gamma-carboxylation in the proteins with the use of a stain specific for Gla residues. Each antibody recognized either BGP or MGP with no cross-reaction between proteins detected. All purified fish BGPs and MGPs tested were shown to be specifically recognized, thus validating the use of these antibodies for further studies.
    • Grant Information:
      AR25921 United States AR NIAMS NIH HHS
    • Molecular Sequence:
      SWISSPROT P83238; P83347; P83473
    • Accession Number:
      0 (Calcium-Binding Proteins)
      0 (Extracellular Matrix Proteins)
      104982-03-8 (Osteocalcin)
    • Publication Date:
      Date Created: 20031212 Date Completed: 20040907 Latest Revision: 20231213
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s00223-003-0079-4
    • Accession Number:
      14668966