[UV spectrophotometric research of drug mixture in sudden poisoning cases].

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  • Additional Information
    • Transliterated Title:
      Vaistu misinio UV-spektrofotometrinis tyrimas ūminiu apsinuodijimu atvejais.
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 9425208 Publication Model: Print Cited Medium: Internet ISSN: 1648-9144 (Electronic) Linking ISSN: 1010660X NLM ISO Abbreviation: Medicina (Kaunas) Subsets: MEDLINE
    • Publication Information:
      Publication: 2018- : Basel, Switzerland : MDPI
      Original Publication: Kaunas : Lietuvos gydytojų sąjunga
    • Subject Terms:
      Amitriptyline/*analysis ; Codeine/*analysis ; Fluoxetine/*analysis ; Poisoning/*diagnosis ; Psychotropic Drugs/*analysis ; Psychotropic Drugs/*poisoning; Acute Disease ; Amitriptyline/poisoning ; Codeine/poisoning ; Data Interpretation, Statistical ; Fluoxetine/poisoning ; Humans ; Research ; Spectrophotometry, Ultraviolet
    • Abstract:
      Unlabelled: The objective of this research--to develop the methodics for analysis of amitriptyline, fluoxetine and codeine in the mixture.
      Results: The analytical method of amitriptyline, codeine and fluoxetine in the mixture identification and quantitative determination using ultraviolet spectrophotometry was established. Preparations in the mixture can't be separated, because material ultraviolet light peaks are in insufficient distance and therefore cover one another. The maximum of ultraviolet light absorption for amitriptyline is at 217-220 and 238-240 nm; fluoxetine--at 226-228 nm; codeine--at 224-248 and 284-286 nm. Using ultraviolet spectroscopy it's possible to identify amitriptyline, fluoxetine and codeine only after separating mixture components by thin-layer chromatography, the same time executing cleaning of extracts from blood and urine. Using ultraviolet spectroscopy can be identificated at least 0.5 micro g/ml amitriptyline, 1.5 micro g/ml fluoxetine and 1.0 microg/ml codeine. The intervals of the quantitative determination: 5-25 microg/ml amitriptyline; 5-30 microg/ml fluoxetine; 100-300 microg/ml codeine; relative error of the measurement, when confidence level is 95%, is from 0.66 to 1.2% for amitriptyline; from 0.66 to 1.45% for fluoxetine; from 0.33 to 0.88% for codeine. Standard deviation is from 1.15 to 2.08% for amitriptyline; from 1.15 to 2.52% for fluoxetine; from 0.57 to 1.53% for codeine. Molar absorption coefficients for all three preparations in distillated water were determinated.
      Conclusions: recommended methodology suits for mixture, extracted from biological liquids, components separation, identification and quantitative determination.
    • Accession Number:
      0 (Psychotropic Drugs)
      01K63SUP8D (Fluoxetine)
      1806D8D52K (Amitriptyline)
      UX6OWY2V7J (Codeine)
    • Publication Date:
      Date Created: 20031118 Date Completed: 20080313 Latest Revision: 20201208
    • Publication Date:
      20231215
    • Accession Number:
      14617875