[Gene therapy of the graft versus host reaction].

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Janousková O;Janousková O; Fales I; Kobylka P; Vonka V
  • Source:
    Casopis lekaru ceskych [Cas Lek Cesk] 2003; Vol. 142 (9), pp. 530-3.
  • Publication Type:
    English Abstract; Journal Article; Research Support, Non-U.S. Gov't; Review
  • Language:
    Czech
  • Additional Information
    • Transliterated Title:
      Genová terapie reakce stĕpu proti hostiteli.
    • Source:
      Publisher: Ceskoslovenska Lekarska Spolecnost Country of Publication: Czech Republic NLM ID: 0004743 Publication Model: Print Cited Medium: Print ISSN: 0008-7335 (Print) Linking ISSN: 00087335 NLM ISO Abbreviation: Cas Lek Cesk Subsets: MEDLINE
    • Publication Information:
      Original Publication: Praha : Ceskoslovenska Lekarska Spolecnost
    • Subject Terms:
      Genetic Therapy*; Graft vs Host Disease/*therapy; Dependovirus ; Genes, Transgenic, Suicide ; Genetic Vectors ; Humans
    • Abstract:
      Graft-versus-host-disease (GVHD) is a frequent and dangerous complication of allogenic transplantations of bone marrow. Gene therapy offers a way to deal with the problem. It is based on the introduction of suicide genes (SG) into the donor's T lymphocytes, which are responsible for the development of GVHD. If it develops, the presence of SG in the effector cells gives an opportunity to get rid of them, because their products are capable of changing otherwise innocuous substances into highly cytotoxic metabolites. For the transduction of SG retrovirus-based vectors are used. The authors tried to employ for this purpose recombinant adeno-associated viruses (rAAV). The attempt was unsuccessful. When using rAAV as vectors, the efficacy of transduction was very low. Further experiments indicated that this failure was due to the absence of receptor for AAV in T lymphocytes. It seems clear that until the surface of rAAV is modified to facilitate their penetration into T cells, they cannot replace retroviruses for transfer of SG into this cell type.
    • Number of References:
      21
    • Publication Date:
      Date Created: 20031112 Date Completed: 20040109 Latest Revision: 20121115
    • Publication Date:
      20231215
    • Accession Number:
      14608943