Comparison of BrdUrd and [3H]TdR incorporation to estimate cell proliferation, cell loss, and potential doubling time in tumor xenografts.

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  • Additional Information
    • Source:
      Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8102328 Publication Model: Print Cited Medium: Print ISSN: 0196-4763 (Print) Linking ISSN: 01964763 NLM ISO Abbreviation: Cytometry Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, N.Y. : Wiley-Liss, c1980-c2002.
    • Subject Terms:
      Bromodeoxyuridine* ; Cell Cycle* ; Cell Death* ; Cell Division* ; Neoplasm Transplantation* ; Thymidine* ; Transplantation, Heterologous* ; Tritium*; Animals ; Autoradiography ; Carcinoma, Squamous Cell/pathology ; DNA, Neoplasm/analysis ; Female ; Humans ; Leg ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Nasal Cavity ; Nose Neoplasms/pathology ; Software ; Tumor Cells, Cultured/transplantation
    • Abstract:
      In this study, two different methods of estimating cell proliferation were compared: cell loss and potential growth rate of xenografted head and neck cancer grown in nude mice based on the detection of DNA incorporation of bromodeoxyuridine (BrdUrd) in one method, and [3H]thymidine ([3H]TdR) in the other. The 21-d-old xenografts were labelled in vivo, either with BrdUrd or [3H]TdR and excised at intervals during 65.5 h. In tumors containing BrdUrd, the percent labelling was measured in mid-S and mid-G1 phase windows of cytograms from bivariate DNA flow cytometry (FCM). In [3H]TdR-labelled tumors, the percent labelled mitoses (PLM) was determined by light microscopy evaluation of autoradiographs. With a computer program based on a theoretical model, the percent labelling versus time after injection was used to analyze cell cycle time, cell loss, tumor growth fraction, and potential doubling time. The values calculated from DNA incorporation with BrdUrd agreed well with those obtained from labelling with [3H]TdR, i.e., cell cycle time 2.3 vs. 2.4 d, and growth fraction 67 vs. 70%. The estimated potential doubling time was 3.1 d and cell loss factor 40% by both methods. Flow cytometry analysis of BrdUrd-labelling is considerably faster than the evaluation of [3H]TdR-labelling, and the present results provide further support for the BrdUrd labelling method as a promising alternative to the PLM method in cell cycle studies designed to evaluate the relevance of cell proliferative properties in relation to biological behavior in xenografted head and neck cancer.
    • Accession Number:
      0 (DNA, Neoplasm)
      10028-17-8 (Tritium)
      G34N38R2N1 (Bromodeoxyuridine)
      VC2W18DGKR (Thymidine)
    • Publication Date:
      Date Created: 19920101 Date Completed: 19930113 Latest Revision: 20131121
    • Publication Date:
      20221208
    • Accession Number:
      10.1002/cyto.990130810
    • Accession Number:
      1459003