Nimesulide inhibits tumor growth in mice implanted hepatoma: overexpression of Bax over Bcl-2.

Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 100956087 Publication Model: Print Cited Medium: Print ISSN: 1671-4083 (Print) Linking ISSN: 16714083 NLM ISO Abbreviation: Acta Pharmacol Sin Subsets: MEDLINE

Publication: 2009- : New York : Nature Publishing Group
Original Publication: Beijing, China : Science Press, c2000-

Apoptosis/*drug effects ; Liver Neoplasms, Experimental/*pathology ; Proto-Oncogene Proteins/*metabolism ; Proto-Oncogene Proteins c-bcl-2/*metabolism ; Sulfonamides/*pharmacology; Animals ; Cyclooxygenase 2 ; Cyclooxygenase 2 Inhibitors ; Cyclooxygenase Inhibitors/pharmacology ; Dinoprostone/metabolism ; Isoenzymes/metabolism ; Liver Neoplasms, Experimental/metabolism ; Male ; Mice ; Neoplasm Transplantation ; Prostaglandin-Endoperoxide Synthases/metabolism ; Random Allocation ; Tumor Cells, Cultured ; bcl-2-Associated X Protein

Aim: To investigate whether nimesulide could suppress tumor growth and induce apoptosis in implanted hepatoma mice and to explore the molecular mechanisms.
Methods: Male mice received nimesulide 10 mg/kg, 20 mg/kg, and 40 mg/kg ig daily for 21 d. Electron microscopy (EM), flow cytometry (FCM), DNA ladder, radioimmunoassay (RIA), and Western blot analysis were employed to investigate effect of nimesulide on mice hepatoma and the related molecular mechanisms.
Results: Nimesulide inhibited the growth of hepatoma (from 14 % to 62 %) and elicited typical apoptotic morphologic changes. The DNA ladder of high dose nimesulide was more clearly observed and apoptotic rate was 51.3 %+/-1.5 %. Nimesulide also decreased cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2) and Bcl-2 expression, while increased the level of Bax protein.
Conclusion: Nimesulide suppresses tumor growth and induces apoptosis by inhibiting COX-2 and PGE2 expression, which may be related to overexpression of Bax over Bcl-2.

0 (Bax protein, mouse)
0 (Cyclooxygenase 2 Inhibitors)
0 (Cyclooxygenase Inhibitors)
0 (Isoenzymes)
0 (Proto-Oncogene Proteins)
0 (Proto-Oncogene Proteins c-bcl-2)
0 (Sulfonamides)
0 (bcl-2-Associated X Protein)
EC 1.14.99.1 (Cyclooxygenase 2)
EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
K7Q1JQR04M (Dinoprostone)
V4TKW1454M (nimesulide)

Date Created: 20031009 Date Completed: 20040805 Latest Revision: 20151119

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