Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH.

Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE

Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology
Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology

Saccharomyces cerevisiae Proteins* ; TATA-Binding Protein Associated Factors* ; Transcription Factor TFIID*; Protein Subunits/*chemistry ; Transcription Factors, TFII/*chemistry; Amino Acid Sequence ; Animals ; Baculoviridae/genetics ; Cyclin-Dependent Kinases/chemistry ; Cyclins/chemistry ; Electrophoresis, Polyacrylamide Gel ; Gene Expression ; Genetic Vectors ; Glutathione Transferase/genetics ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; Recombinant Fusion Proteins ; Saccharomyces cerevisiae ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Spodoptera/metabolism ; Transcription Factor TFIIH ; Transcription Factors, TFII/genetics ; Transcription Factors, TFII/isolation & purification

Tfb4 is identified as a subunit of the core complex of yeast RNA polymerase II general transcription factor IIH (TFIIH) by affinity purification, by peptide sequence analysis, and by expression of the entire complex in insect cells. Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin subunits of TFIIH. This reassignment of subunits resolves a longstanding discrepancy between yeast and human TFIIH complexes.

GM36659 United States GM NIGMS NIH HHS; P30 CA08748 United States CA NCI NIH HHS

0 (Cyclins)
0 (Protein Subunits)
0 (Recombinant Fusion Proteins)
0 (Saccharomyces cerevisiae Proteins)
0 (TAF6 protein, S cerevisiae)
0 (TATA-Binding Protein Associated Factors)
0 (Transcription Factor TFIID)
0 (Transcription Factors, TFII)
148710-81-0 (Transcription Factor TFIIH)
EC 2.5.1.18 (Glutathione Transferase)
EC 2.7.11.22 (Cyclin-Dependent Kinases)

Date Created: 20030923 Date Completed: 20040105 Latest Revision: 20210206

20221213

10.1074/jbc.C300417200

14500720