A cikta juh jellemzése a mitokondriális DNS kontrollrégiója alapján. (Hungarian)

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Alternate Title:
      Characterisation of Hungarian Cikta sheep based on the control region of mtDNA. (English)
    • Abstract:
      Background: The consideration of the high genetic diversity is indispensable on the course of preservation of endangered animal breeds. Objectives: The authors evaluate the genetic background in the Hungarian native Cikta breed by use of mitochondrial DNA (mtDNA) control region (CR) sequence firstly. Their investigation was carried out in order to serve data for the maintenance of maternal lineages. Materials and Methods: The DNA samples were taken from the descendants of the eldest families by use of founder sampling method based on pedigree (n = 69) in 2015. The primers described by Hiendleder et al. (7) were used to amplify the region of interest (AF010406). Results and Discussion: The control region of mtDNA showed polymorphisms at 32 sites. However, the herds shared 24 polymorphic sites, so the maternal background of the Cikta appears to be genetically uniform. The total number of haplotypes were 13, furthermore, most of the samples belonged to the haplogroup B of sheep. This fact proves the decisively European maternal origin of the Hungarian Cikta. The average number of pairwise differences (k) and the average nucleotide diversity (p) were 6.863 and 5.95 × 10-3, respectively. The values of the Cikta population were not significant (p < 0.10) neither by the Tajima D-test (0.107) and by Fu's Fs statistics (2.533), meaning that the greatly reduced population size of the breed known from the breed history did not cause genetic drift, it is in genetic equilibrium regarding its ancient families. The genetic information confirmed the origin of the families/flocks known from the breed history. A more intense focusing on the maternal side is motivated also by the fact that the females are present at greater number than the males, respectively they remain in breeding for a longer period of time, so they can at larger extent be the depositaries of realization and maintenance of genetic diversity. [ABSTRACT FROM AUTHOR]
    • Abstract:
      A veszélyeztetett állatfajták fenntartásában elengedhetetlen a genetikai változatosság figyelembevétele. A szerzők célja, a fajtában elsőként, az őshonos cikta anyai genetikai hátterének mitokondriális DNS (mtDNS) szekvencia alapján történő vizsgálata a családok és haplotípusok megőrzése érdekében. A DNS-mintákat a törzskönyvileg igazolt legrégebbi családok leszármazottjaitól gyűjtötték (n = 69) 2015-ben. A mtDNS kontrollrégiója 32 helyen mutatott nukleotideltérést, ugyanakkor a nyájak 24 polimorf helyen osztoznak egymással, tehát a cikta anyai háttere genetikailag egységesnek tűnik. A genetikai információ nagyban vis?- szaigazolta a családok/nyájak fajtatörténetből ismert eredetét. A minták többsége a juh B haplocsoportjához tartozott, ami a magyarországi cikta számottevő európai anyai származását igazolja. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Magyar Állatorvosok Lapja is the property of Herman Otto Intezet Nonprofit Kft. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)