PML isoform expression and DNA break location relative to PML nuclear bodies impacts the efficiency of homologous recombination.

DNA repair; SISTER chromatid exchange; POST-translational modification; UBIQUITIN ligases; DNA; BACTERIAL artificial chromosomes; MAMMALIAN cell cycle; GENE transfection

The article discusses that Promyelocytic leukemia nuclear bodies (PML NBs) as nuclear subdomains that respond to genotoxic stress by increasing in number via changes in chromatin structure. It mentions the role of the PML protein and PML NBs in specific mechanisms of DNA repair, along with examined the role of PML in homologous recombination (HR) using I-SceI extrachromosomal and chromosome-based homology-directed repair (HDR) assays, and in HDR by CRISPR/Cas9-mediated gene editing.