EFFECT OF DIET ON THE ACTIVITY OF SEVERAL ENZYMES IN EXTRACTS OF RUMEN MICROORGANISMS.

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  • Author(s): BALDWIN RL; PALMQUIST DL
  • Source:
    Applied microbiology [Appl Microbiol] 1965 Mar; Vol. 13, pp. 194-200.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 7605802 Publication Model: Print Cited Medium: Print ISSN: 0003-6919 (Print) Linking ISSN: 00036919 NLM ISO Abbreviation: Appl Microbiol Subsets: OLDMEDLINE
    • Publication Information:
      Original Publication: Washington DC : American Society For Microbiology
    • Subject Terms:
      Acyltransferases* ; Bacteria* ; Biochemical Phenomena* ; Carbohydrate Metabolism* ; Diet* ; Dihydrolipoamide Dehydrogenase* ; Edible Grain* ; Enzymes* ; Fermentation* ; Fructose-Bisphosphate Aldolase* ; Hydro-Lyases* ; L-Lactate Dehydrogenase* ; Lipid Metabolism* ; Malate Dehydrogenase* ; Metabolism* ; Oxidoreductases* ; Pharmacology* ; Research* ; Rumen* ; Spectrophotometry* ; Succinate Dehydrogenase*; Fatty Acids/*metabolism ; Glucose/*metabolism; Animals ; Cattle ; Female
    • Abstract:
      The use of enzymatic techniques to characterize rumen metabolism was investigated. Assays were developed to estimate the activities of 14 enzymes in cell-free extracts of microorganisms collected from rumen contents of cows fed two diets, selected to produce widely different proportions of fermentation end products. The results reflected the differences between the two diets in metabolic potential, fermentation patterns, and microbial populations. The differences between the diets in the relative activities of succinic dehydrogenase and fumaric reductase, for example, indicated a shift in the microbial population favoring organisms of the Viellonella alcalescens type on the concentrate diet. The data presented indicate that, if employed carefully, enzymatic criteria can be utilized effectively in studies of rumen metabolism.
    • References:
      J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713)
      Arch Biochem Biophys. 1959 Jun;82(2):274-87. (PMID: 13661952)
      Bacteriol Rev. 1960 Dec;24(4):353-64. (PMID: 13716827)
      J Gen Microbiol. 1962 Apr;28:119-28. (PMID: 14040171)
      J Bacteriol. 1963 Jun;85:1346-9. (PMID: 14047228)
      Bacteriol Rev. 1959 Sep;23(3):125-53. (PMID: 13805451)
    • Contributed Indexing:
      Keywords: ACYLTRANSFERASES*; ALDOLASE*; BACTERIA*; CATTLE*; DIET*; ENZYMES*; EXPERIMENTAL LAB STUDY*; FATTY ACID METABOLISM*; FERMENTATION*; GLUCOSE METABOLISM*; GRAIN*; HYDRO-LYASES*; LACTATE DEHYDROGENASE*; LIPOAMIDE DEHYDROGENASE*; MALATE DEHYDROGENASE*; METABOLISM*; OXIDOREDUCTASES*; PHARMACOLOGY*; RUMEN*; SPECTROPHOTOMETRY*; SUCCINATE DEHYDROGENASE*
    • Accession Number:
      0 (Enzymes)
      0 (Fatty Acids)
      EC 1.- (Oxidoreductases)
      EC 1.1.1.27 (L-Lactate Dehydrogenase)
      EC 1.1.1.37 (Malate Dehydrogenase)
      EC 1.3.99.1 (Succinate Dehydrogenase)
      EC 1.8.1.4 (Dihydrolipoamide Dehydrogenase)
      EC 2.3.- (Acyltransferases)
      EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
      EC 4.2.1.- (Hydro-Lyases)
      IY9XDZ35W2 (Glucose)
    • Publication Date:
      Date Created: 19650301 Date Completed: 19961201 Latest Revision: 20210526
    • Publication Date:
      20221213
    • Accession Number:
      PMC1058220
    • Accession Number:
      10.1128/am.13.2.194-200.1965
    • Accession Number:
      14325878