Phenotypic Variation in Growth and Gene Expression Under Different Photoperiods in Allopatric Populations of the Copepod Tigriopus californicus.

Daylength is a major environmental condition that varies seasonally and predictably along a latitudinal cline, where higher latitudes exhibit greater ranges in total daylengths. Generally, the circadian clock acts as a network of genes whose expression dynamics are known to control daily rhythms in response to daylength, and it enables the control of many physiological processes such as growth and development. While well studied in many model animals, the influence of daylength variation on phenotypic evolution is poorly examined in marine species. In this study we demonstrate that two allopatric populations of the intertidal crustacean Tigriopus californicus exhibit plastic and divergent phenotypic responses to changes in daylength. Using common-garden experiments, we discovered that shorter daylengths promoted decreased adult body size and faster growth rates in the two divergent populations, suggesting a plastic response to shortened days. In addition, the higher-latitude population exhibited a faster growth rate at any daylength condition, indicating a fixed response, possibly as a result of adaptation to respective natural light regimes. Gene expression profiles of several circadian clock genes, monitored throughout the day by quantitative polymerase chain reaction, revealed that the key core clock genes reach higher daily transcription maxima in the southern population compared to the northern population, pointing to divergent strategies used to respond to changes in daylength. Many modifier genes to the circadian clock showed similar plastic responses to the different daylengths, supporting the existence of at least some conserved gene expression across both populations. Ultimately, our results suggest that photoperiod and daylength exert a potent selective pressure underexplored in marine systems and warranting further future research. [ABSTRACT FROM AUTHOR]

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