AVALIAÇÃO DA ATIVIDADE ANTIFÚNGICA DOS EXTRATOS ETANÓLICOS DO MELÃO-DE-SÃOCAETANO (Momordica charantia L.) FRENTE A DIFERENTES ESPÉCIES DE CANDIDA.

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    • Alternate Title:
      Evaluation of the antifungal activity of ethanolic extracts of the bitter melon (Momordica Charantia L.) Against different species of candida.
    • Subject Terms:
      WATERMELONS; MOMORDICA charantia; RESEARCH ethics; FRUIT extracts; RURAL geography; CANDIDA; CANDIDEMIA; ANTIFUNGAL agents
    • Abstract:
      Introduction: Yeast of the candida gender are mostly related to etiology of mycosis. Objective: evaluate the antifungal activity of ethanolic extracts of the Bitter melon (Momordica charantia L.) against different species of Candida. Methodology: after the approval by the Research Ethics Committee, fruits and leaves of the Bitter melon were collected in the rural area of Bauru-SP. The fruits and leaves were taken to a greenhouse and kept with forced air circulation for 96 hours at the temperature of 45 °C. After that period, the samples were submitted to a grinding process in turbolysis. The grindings were weighed and each 10g received 85mL of alcohol 70%. The macerates were stored in an Erlenmeyer flask and were kept at rest for 21 days; after that, they were appropriately packed so that no light would penetrate in the solution. In the sequence, the liquid suffered a vacuum filtration and the solvent was evaporated in a hood to obtain the ethanolic extracts. The tests were performed in triplicate. Results: The extract of the fruit did not show fungistatic (CIM) nor fungicidal capacity (CFM) in the maximum concentration evaluated (1250µg/mL) against the species of Candida used. On the other hand, the extract of the leave showed fungistatic activity (CIM) against the other species of Candida, presenting different patterns in the concentrations (312.5µg/mL for C. albicans and 625µg/mL for C. krusei, C. tropicalis and C. glabrata). The same extract showed fungicidal capacity (CFM) in the species C. albicans and C. tropicalis, in the concentrations 625µg/mL and 1250µg/mL, respectively. Conclusion: the extract of the leave of the Bitter melon was effective against all Candida strains studied, suggesting this is a great therapeutic alternative. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Introdução: Leveduras do gênero Candida são reconhecidas por serem as espécies mais comuns envolvidas na etiologia das micoses. Objetivo: avaliar a atividade antifúngica dos extratos etanólicos do Melão-de-São-Caetano (Momordica charantia L.) frente a diferentes espécies de Candida. Metodologia: após aprovação pelo Comitê de Ética em Pesquisa, a coleta dos frutos e das folhas do Melão-de-SãoCaetano foi realizada na região da zona rural de Bauru-SP. Os frutos in natura e as folhas foram levados à estufa com circulação forçada de ar por 96 horas a 45°C, sendo, após esse período, submetidos ao processo de moagem em turbólise. Os triturados foram pesados e a cada 10g foram acrescentados 85mL de álcool 70%. Os macerados foram armazenados em erlemeyer, ficaram em repouso por 21 dias, foram devidamente embalados para que a luz não penetrasse na solução. Na sequência, o líquido foi filtrado a vácuo e o solvente evaporado em capela para obtenção dos extratos etanólicos. Os testes foram realizados em triplicata. Resultados: o extrato do fruto não apresentou capacidade fungistática (CIM) nem fungicida (CFM) na máxima concentração avaliada (1250µg/mL) sob as espécies de Candida utilizadas. Já o extrato da folha demonstrou ação fungistática (CIM) frente a todas as espécies de Candida, mostrando padrões diferentes nas concentrações (312,5µg/mL para C. albicans e 625µg/ mL para C. krusei, C. tropicalis e C. glabrata). O mesmo extrato demonstrou capacidade fungicida (CFM) nas espécies C. albicans e na C. tropicalis, nas concentrações 625µg/mL e 1250µg/mL respectivamente. Conclusão: o extrato da folha do Melão-de-SãoCaetano foi eficaz sobre todas as linhagens de Candida ensaiadas, sugerindo uma ótima alternativa terapêutica. [ABSTRACT FROM AUTHOR]
    • Abstract:
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