The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury.

Objective: The effects of urapidil in intestinal ischemia-reperfusion (IR) model were investigated using histopathological and biochemical methods. Materials and methods: Forty Wistar albino rats were subjected to sham operation (Group 1), IR (Group 2), IR+dimethyl sulfoxide (Group 3), IR+urapidil 0.5 mg/kg (Group 4), and IR+urapidil 5 mg/kg (Group 5). Levels of MDA, TAS, TOS, SOD, MPO, NF-κB, caspase-3, and LC3B were measured. Results and discussion: The groups 2 and 3 had significantly higher TOS and MPO levels than the sham group had (p < 0.001), whereas the TAS and SOD levels were significantly lower in Group 2 than in the sham group. In treatment groups, TAS and SOD levels increased, whereas TOS, MPO, and MDA levels decreased compared to Group 2. Caspase-3 and LC3B immunopositivities were seen at severe levels in Group 2 and 3. However, Group 4 and 5 were found to have lower levels of immunopositivity. Immunopositivity was observed in interstitial areas, peribronchial region, and bronchial epithelial cells. A moderate level of NF-κB immunopositivity was seen in Group 2 and 3. Conclusion: Our results show that urapidil is one of the antioxidant agents and protects lung tissue from oxidant effects of intestinal IR injury. [ABSTRACT FROM AUTHOR]

Öz: Amaç İntestinal iskemi-reperfüzyon (IR) modelinde urapidil'in etkileri histopatolojik ve biyokimyasal yöntemler kullanılarak araştırıldı. Gereç ve Yöntem Kırk adet Wistar albino sıçan sham operasyon (Grup 1), IR (Grup 2), IR+dimetil sülfoksit (Grup 3), IR+urapidil 0.5 mg/kg (Grup 4) ve IR+urapidil 5 mg/kg (Grup 5) olarak gruplandı. MDA, TAS, TOS, SOD, MPO, NF-κB, kaspaz-3 ve LC3B seviyeleri ölçüldü. Bulgular ve tartışma Grup 2 ve 3'te sham grubuna göre TOS ve MPO düzeylerinin anlamlı derecede yüksek, (p < 0.001), TAS ve SOD düzeyleri grup 2'de sham grubundan anlamlı derecede düşüktü. Tedavi gruplarında TAS ve SOD seviyeleri yükselirken, TOS, MPO ve MDA düzeyleri Grup 2'ye göre arttı. Kaspaz-3 ve LC3B immünopozitivitesi Grup 2 ve 3'te yoğun seviyelerde görüldü İnterstisyel bölgelerde, peribronşiyal bölgede ve bronşiyal epitel hücrelerinde immünopozitivite gözlendi. Grup 2 ve 3'te orta düzeyde bir NF-kappa B immünopozitivitesi görüldü. Sonuç Sonuçlarımız urapidil'in, antioksidan ajanlardan biri olduğu ve akciğer dokusunu bağırsak IR hasarının oksidan etkilerinden koruduğunu göstermiştir. [ABSTRACT FROM AUTHOR]

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