The effect of ascorbic acid oxidation on the incorporation of sulfate by slices of calf costal cartilage.

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  • Author(s): KLEBANOFF SJ; DZIEWIATKOWSKI DD; OKINAKA GJ
  • Source:
    The Journal of general physiology [J Gen Physiol] 1958 Nov 20; Vol. 42 (2), pp. 303-18.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 2985110R Publication Model: Print Cited Medium: Print ISSN: 0022-1295 (Print) Linking ISSN: 00221295 NLM ISO Abbreviation: J Gen Physiol Subsets: OLDMEDLINE
    • Publication Information:
      Publication: New York, N.Y. : Rockefeller University Press
      Original Publication: New York, N.Y. : Rockefeller Institute for Medical Research, c1918-
    • Subject Terms:
      Copper* ; Costal Cartilage*; Ascorbic Acid/*pharmacology ; Cartilage/*metabolism ; Ribs/*metabolism ; Sulfates/*metabolism; Animals ; Cattle
    • Abstract:
      A marked inhibition of the incorporation of S(35)-sulfate by normal calf costal cartilage was produced by potassium ascorbate in the presence of catalytic amounts of cupric ions. The effect of the various components of the ascorbic acid oxidizing system (potassium ascorbate, cupric ions, cuprous ions, hydrogen peroxide, dehydroascorbic acid) was investigated. The results of experiments in which hydrogen peroxide, catalase, or sodium azide were used singly or in combination suggest that the inhibition produced by the ascorbic acid oxidizing system is due, to a considerable extent, to the production of hydrogen peroxide. Dehydroascorbic acid was also found to inhibit the incorporation of S(35)-sulfate by cartilage slices. However, the gradual fall in pH which resulted from the addition of dehydroascorbic acid could account to a large extent for the inhibitory effect observed because the incorporation of S(35)-sulfate by cartilage slices decreases sharply as the pH is lowered. The incorporation of S(35)-sulfate by cartilage slices is inhibited also by increasing the concentration of phosphate.
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    • Contributed Indexing:
      Indexing Agency: CLML Local ID #: 5935:17323:121:518:564:625.
      Non-Mesh Headings: CARTILAGE/metabolism*; RIBS/metabolism*; SULFATES/metabolism*; VITAMIN C/effects*
    • Accession Number:
      0 (Sulfates)
      17493-86-6 (cuprous ion)
      789U1901C5 (Copper)
      PQ6CK8PD0R (Ascorbic Acid)
    • Publication Date:
      Date Created: 19581120 Date Completed: 20000701 Latest Revision: 20190509
    • Publication Date:
      20250114
    • Accession Number:
      PMC2194906
    • Accession Number:
      10.1085/jgp.42.2.303
    • Accession Number:
      13587914