細胞接着剤の調製と応用. (Japanese)

Design and Application of Cell Glue. (English)

Since amphiphilic polymers carrying adhesive domains can be used for cell surface modification, they induce cell adhesion by acting as cell glues. For example, conjugation or single stranded DNA (ssDNA) or cell-penetrating peptide to poly(ethylene glycol)-conjugated phospholipid (PEG-lipid) made it possible to induce cell-cell attachment and cell-substrate attachment. These PEG-lipid derivatives are very convenient for this purpose because they are not taken up into cells and are localized on the cell surface, which means that the adhesive domains are displayed on the cell surface. In future our PEG-lipid derivatives will be useftil for cell-based arrays for drug screening and high-throughput gene analyses, and pancreatic islet transplantation and regenerative medicine which require 3D tissue structures. [ABSTRACT FROM AUTHOR]

親媒性高分子に機能性部位（ここでは， 粘着 ドメインとし作用する機能性部位）を導入した材料は， 細 胞膜の脂質二重層との弱い疎水性相互作用により細胞表面 を修飾できるため， 細胞を自在に接着できる細胞接着剤と して利用できる.ポリエチレングリコール結合脂質（PEG 脂質)へ， 短鎖DNA (ssDNA) や膜透過性ペプチド（CPP) を 結合させることで， これまでは不可能であった細胞-細胞 どうしの接着や細胞-基板間の接着を実現できる.この PEG脂質誘導体は細胞機能を阻害せず， また細胞内部へ取 り込まれず， 細胞表面上にのみこれらの粘着ドメインを提 示できるため， 細胞接着剤として有用である.今後， 細胞 表面工学を応用したこの細胞接着剤は， 薬剤のスクリーニ ングや遺伝子機能解析のハイスル一プットアツセイのため の細胞アレイ作成や1型糖尿病への脖島移植， あるいは再 生医療のための三次元組織構築への応用が期待できる. [ABSTRACT FROM AUTHOR]

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