健康成年人血清α-L-岩藻糖苷酶参考区间的建立及适用性评价. (Chinese)

Establishment and Applicability Evaluation of Reference Interval for Serum AFU of Healthy Adults. (English)

Objective To establish the reference interval of serum α-L-fucosidase (AFU) in healthy adults and evaluate the clinical application of AFU. Methods A total of 480 healthy adult physical examinees from April to October 2017 in Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University were Collected. There were 240 males and 240 females by sex. They were divided into youth group (18 ~44 years old) 160 cases, middle age group (45 ~ 59 years old) 160 cases and elderly group (60 years old or above 60 years old) 160 caces according to age. At the same lime, 119 cases of primary liver cancer were collected as case group,including 91 males,28 women and 24~82 years old. The results of serum AFU were measured by CNPF substrate method, and the data were statistically analyzed. Results AFU levels of the male group, the young group,the middle-aged group,the elderly group,the young male group,the young female group,the middle-aged male group, the middle-aged female group, the elderly male group and the elderly female group were 26. 39±6. 55,25. 23±6. 31,24. 18± 5. 78,26. 80±6. 79,26. 47±6. 47,24. 84 ± 5. 56,23. 53±5. 96,27. 29±7. 18,26. 27±6. 38,27. 03±6. 62 and 25. 91 ±6. 31 U/L,respectively. There was no significant difference in serum AFU levels between different ages and sex (P>0. 05). The single reference interval of serum AFU in healthy adults ranged from 13U/L to 38 U/L. The sensitivity and specificity of this interval for primary liver cancer patients were 83. 2% and 94. 1% respectively. Conclusion The reference interval of serum AFU in healthy adults has been established through research,providing more accurate experimental evidence for clinical diagnosis and treatment of primary liver cancer. In a word.AFU has a better clinical application in hepatopathy. [ABSTRACT FROM AUTHOR]

目的 建立健康成年人血清α-L-岩藻糖苷酶(α-L-fucosidase,AFU)参考区间,并对AFU的临床适用性进行评价。方法 收集中山大学孙逸仙纪念医院2017年4~10月480例健康成年体检者,其中按性别分为男性组240例,女性组240例;按年龄分为青年组(18~44岁)160例、中年组(45~59岁)160例和老年组(≥60岁)160例,各个年龄组再按性别分组。同时收集确诊为原发性肝癌的患者119例作为病例组,其中男性91例,女性28例,年龄24~82岁,采用CNPF底物法测定其血清AFU结果,并对数据进行统计学分析。结果 男性组、女性组、青年组、中年组、老年组、青年男性组、青年女性组、中年男性组、中年女性组、老年男性组和老年女性组的AFU水平分别为26.39±6.55,25.23±6.31,24.18±5.78,26.80±6.79,26.47±6.47,24.84±5.56,23.53±5.96,27.29±7.18,26.27±6.38,27.03±6.62和25.91±6.31U/L。不同年龄及性别间血清AFU水平的差异均无统计学意义(P>0.05)。健康成年人血清AFU的单一参考区间为13~38U/L。用此区间评估原发性肝癌患者的灵敏度为83.2%,特异度为94.1%。结论 通过研究建立了健康成年人血清AFU的参考区间,为临床原发性肝癌的诊断和治疗提供了更加准确的实验依据。AFU在肝病患者中具有较高的临床适用价值。 [ABSTRACT FROM AUTHOR]

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