Dual probing of redox species, NAD(P)H and HOCl, with a benzo[a]phenoxazine based far red-emitting dye.

We report a benzo[ a ]phenoxazine-based far red-emitting probe which selectively senses reduced nicotinamide adenine dinucleotide (NADH) and its phosphate ester NADPH, the most indispensable coenzymes found in all living cells, with “ turn-off ” fluorescence response at 650 nm. The original probe can be regenerated quantitatively through oxidation using hypochlorous acid, a reactive oxygen species, which was accompanied by restored fluorescence at 650 nm. This probe was also applied for fluorescence imaging of NADH in HeLa cells. [ABSTRACT FROM AUTHOR]

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