Coumarin/BODIPY Hybridisation for Ratiometric Sensing of Intracellular Polarity Oscillation.

Abstract: With different polarity responses, coumarin and 4,4‐difluoro‐4‐bora‐3a,4a‐diaza‐s‐indacene (BODIPY) were hybridised to construct polarity fluorescent sensors, CBDP and iso‐CBDP, to overcome the disadvantages of solvatochromic sensors in ratiometric polarity sensing. Only CBDP displayed an emission ratio (ICou/IBDP, coumarin to BODIPY emissions) that increased with an exponential dependence on medium relative permittivity over a wide polarity range (ϵr<57.9). This sensing ability of CBDP was not affected by medium pH; viscosity; and most intracellular species, especially reactive oxygen, nitrogen, and sulfur species. Apart from local cytoplasmic polarity quantification through lambda imaging, CBDP enables real‐time ratiometric imaging for intracellular polarity oscillation induced by oxidative stimulation. Ratiometric polarity flow cytometry was developed, for the first time, with CBDP, which demonstrated that a high concentration H2O2 induced cytoplasmic polarity enhancement, whereas pre‐incubation with N‐acetyl‐ l‐cysteine inhibited this effect. [ABSTRACT FROM AUTHOR]

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