Cultivation and properties of Echinamoeba thermarum n. sp., an extremely thermophilic amoeba thriving in hot springs.

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  • Author(s): Baumgartner M;Baumgartner M; Yapi A; Gröbner-Ferreira R; Stetter KO
  • Source:
    Extremophiles : life under extreme conditions [Extremophiles] 2003 Aug; Vol. 7 (4), pp. 267-74. Date of Electronic Publication: 2003 Mar 28.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: Germany NLM ID: 9706854 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1431-0651 (Print) Linking ISSN: 14310651 NLM ISO Abbreviation: Extremophiles Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin : Springer, c1997-
    • Subject Terms:
      Hot Temperature* ; Phylogeny*; Amoebida/*classification ; Amoebida/*growth & development ; Water/*parasitology; Amoebida/cytology ; Amoebida/isolation & purification ; Animals ; Costa Rica ; Hydrogen-Ion Concentration ; Italy ; Microscopy, Electron, Scanning ; RNA, Protozoan/genetics ; RNA, Ribosomal/genetics ; Russia ; Sodium Chloride/metabolism ; United States
    • Abstract:
      Here we describe a new, extremely thermophilic amoeba growing between 33 degrees C and 57 degrees C ( Topt.=50 degrees C). Isolates had been obtained from hot springs at Agnano Terme (Italy), Yellowstone National Park (USA), Kamchatka (Russia), and the Arenal Volcano (Costa Rica). They could be cultured monoxenically on a thermophilic alpha-proteobacterium. The morphology of the amoeba was studied using a microscope situated under a heatable polyacrylate hood. At 50 degrees C, the cells appeared flat with an irregular triangular or elongate shape, sometimes exhibiting fine spine-like subpseudopodia. On average, they were 22 microm long and 11 microm wide and had one nucleus with a central nucleolus. Based on morphology and on SSU rRNA comparisons, the amoeba belonged to the genus Echinamoeba, where it represents a new species. Referring to its extremely thermophilic lifestyle and its hydrothermal habitat, we name it E. thermarum.
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    • Accession Number:
      0 (RNA, Protozoan)
      0 (RNA, Ribosomal)
      059QF0KO0R (Water)
      451W47IQ8X (Sodium Chloride)
    • Publication Date:
      Date Created: 20030812 Date Completed: 20040601 Latest Revision: 20181113
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s00792-003-0319-6
    • Accession Number:
      12910386