[Rapid laboratory detection of antigens of infective agents of infections and technical means for their realization].

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  • Author(s): Kal'noĭ SM;Kal'noĭ SM
  • Source:
    Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2003 Mar-Apr (2), pp. 51-8.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    Russian
  • Additional Information
    • Transliterated Title:
      Uskorennaia laboratornaia indikatsiia antigenov vozbuditeleĭ osobo opasnykh infektsiĭ i tekhnicheskie ustroĭstva dlia ee obespecheniia.
    • Source:
      Publisher: FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora Country of Publication: Russia (Federation) NLM ID: 0415217 Publication Model: Print Cited Medium: Print ISSN: 0372-9311 (Print) Linking ISSN: 03729311 NLM ISO Abbreviation: Zh Mikrobiol Epidemiol Immunobiol Subsets: MEDLINE
    • Publication Information:
      Publication: <2018, no. 4 (ii︠u︡lʹ-avgust)- >: Moskva : FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora
      Original Publication: Moskva : Izd-vo "Medit︠s︡ina"
    • Subject Terms:
      Hemagglutination Tests* ; Latex Fixation Tests*; Antigens, Bacterial/*blood ; Centrifugation/*instrumentation ; Densitometry/*instrumentation; Brucellosis/blood ; Cholera/blood ; Patents as Topic ; Plague/blood ; Russia ; Time Factors ; Tularemia/blood
    • Abstract:
      A system of new accelerated and rapid methods for the detection of the antigens of the infective agents of plague, cholera, tularemia and brucellosis were developed on the basis of solid phase immunosuspension tests: the passive hemagglutination (PHA) test and the latex agglutination (LA) test. The immunological and physico-chemical properties of suspensions in the PHA and LA tests made it possible to use extraneous sources of energy (centrifugal acceleration and the electric field) to accelerate these tests. The results of the PHA and LA tests were registered with the use of a densitometer, model Ultrascan 2202, and a tester, model C 34014.2. To apply centrifugal acceleration and the electric field, a laboratory centrifuge and an electrophoretic microchamber were designed. Densitometry was carried out on modified plates and conductometry, with the use of modified electrodes. The time of obtaining the results of the PHA and LA tests was 15-30 minutes with the use of centrifugation and 2-5 minutes in the electric field, which made it possible to regard these tests as rapid.
    • Accession Number:
      0 (Antigens, Bacterial)
    • Publication Date:
      Date Created: 20030731 Date Completed: 20031208 Latest Revision: 20200716
    • Publication Date:
      20221213
    • Accession Number:
      12886608