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Analytical exclusion chromatography.
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- Author(s): Winzor DJ;Winzor DJ
- Source:
Journal of biochemical and biophysical methods [J Biochem Biophys Methods] 2003 Jun 30; Vol. 56 (1-3), pp. 15-52.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't; Review
- Language:
English
- Additional Information
- Source:
Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7907378 Publication Model: Print Cited Medium: Print ISSN: 0165-022X (Print) Linking ISSN: 0165022X NLM ISO Abbreviation: J Biochem Biophys Methods Subsets: MEDLINE
- Publication Information:
Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
- Subject Terms:
- Abstract:
This review summarizes the development of exclusion chromatography, also termed gel filtration, molecular-sieve chromatography and gel permeation chromatography, for the quantitative characterization of solutes and solute interactions. As well as affording a means of determining molecular mass and molecular mass distribution, the technique offers a convenient way of characterizing solute self-association and solute-ligand interactions in terms of reaction stoichiometry and equilibrium constant. The availability of molecular-sieve media with different selective porosities ensures that very little restriction is imposed on the size of solute amenable to study. Furthermore, access to a diverse array of assay procedures for monitoring the column eluate endows analytical exclusion chromatography with far greater flexibility than other techniques from the viewpoint of solute concentration range that can be examined. In addition to its widely recognized prowess as a means of solute separation and purification, exclusion chromatography thus also possesses considerable potential for investigating the functional roles of the purified solutes.
- Number of References:
128
- Accession Number:
0 (Biopolymers)
0 (Enzymes)
0 (Solutions)
- Publication Date:
Date Created: 20030702 Date Completed: 20040521 Latest Revision: 20190916
- Publication Date:
20221213
- Accession Number:
10.1016/s0165-022x(03)00071-x
- Accession Number:
12834967
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