Diagnosis of histoplasmosis by detection of the internal transcribed spacer region of fungal rRNA gene from a paraffin-embedded skin sample from a dog in Japan.

Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print Cited Medium: Print ISSN: 0378-1135 (Print) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE

Original Publication: Amsterdam, Elsevier Scientific Pub. Co.

DNA, Ribosomal Spacer/*analysis ; Dog Diseases/*diagnosis ; Histoplasma/*isolation & purification ; Histoplasmosis/*veterinary ; RNA, Fungal/*genetics; Animals ; Base Sequence ; DNA, Ribosomal Spacer/chemistry ; DNA, Ribosomal Spacer/genetics ; Dog Diseases/microbiology ; Dogs ; Female ; Histoplasma/genetics ; Histoplasmosis/diagnosis ; Histoplasmosis/microbiology ; Japan ; Macrophages/microbiology ; Molecular Sequence Data ; Polymerase Chain Reaction/methods ; Polymerase Chain Reaction/veterinary ; RNA, Fungal/isolation & purification ; RNA, Ribosomal, 5.8S/genetics ; Sequence Homology, Nucleic Acid ; Skin/microbiology ; Skin/pathology

The lesions of histoplasmosis in dogs in Japan differ from those in dogs in North America. Affected dogs in Japan have had multiple granulomatous or ulcerated foci in skin or gingiva and have not had pulmonary or gastrointestinal lesions. The present report introduces a polymerase chain reaction (PCR) diagnosis of canine histoplasmosis and the characteristic of disease in Japan. The surgically removed skin ulcerate samples from a 5-years-old female Shiba-inu native to Japan without traveling out of the country were evaluated. Tissue samples had many yeast-like organisms in the macrophages. DNA was extracted from paraffin-embedded tissue samples. A nested PCR technique was applied. The detected sequence of the internal transcribed spacer of ribosomal RNA gene had 99.7% in homology with Ajellomyces capsulatus (the teleomorph of Histoplasma capsulatum). Clinical manifestations, historical background of equine epizootic lymphangitis in Japan, and a human autochthonous case of histoplasmosis farciminosi indicated that this dog might have been infected with H. capsulatum var. farciminosum as a heteroecism.

GENBANK AB101435

0 (DNA, Ribosomal Spacer)
0 (RNA, Fungal)
0 (RNA, Ribosomal, 5.8S)

Date Created: 20030620 Date Completed: 20031006 Latest Revision: 20190901

20231215

10.1016/s0378-1135(03)00104-4

12814889