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Effects of propofol on the activity of rat glutamate transporter type 3 expressed in Xenopus oocytes: the role of protein kinase C.
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- Author(s): Do SH;Do SH; Ham BM; Zuo Z
- Source:
Neuroscience letters [Neurosci Lett] 2003 Jun 05; Vol. 343 (2), pp. 113-6.
- Publication Type:
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: Elsevier Scientific Publishers Ireland Country of Publication: Ireland NLM ID: 7600130 Publication Model: Print Cited Medium: Print ISSN: 0304-3940 (Print) Linking ISSN: 03043940 NLM ISO Abbreviation: Neurosci Lett Subsets: MEDLINE
- Publication Information:
Publication: Limerick : Elsevier Scientific Publishers Ireland
Original Publication: Amsterdam, Elsevier/North-Holland.
- Subject Terms:
- Abstract:
We investigated the effects of propofol on one type of glutamate transporter, excitatory amino acid transporter 3 (EAAT3) and the role of protein kinase C (PKC) in mediating these effects. Rat EAAT3 was expressed in Xenopus oocytes. L-glutamate (30 microM)-induced membrane currents were measured. Propofol increased glutamate-induced inward currents significantly at two tested concentrations (30 and 100 microM) but not at other concentrations. Propofol (30 microM) significantly increased V(max), but not K(m) of EAAT3 for glutamate. The combination of phorbol-12-myrisate-13-acetate (PMA, a PKC activator) and propofol did not increase the responses further compared with PMA or propofol alone. Three PKC inhibitors (staurosporine, calphostin C, and chelerythrine) did not affect basal EAAT3 activity but significantly inhibited the propofol-enhanced EAAT3 activity. Our results suggest that propofol enhances EAAT3 activity at clinically relevant concentrations and PKC may mediate these effects.
- Accession Number:
0 (Amino Acid Transport System X-AG)
0 (Anesthetics, Intravenous)
0 (Enzyme Inhibitors)
0 (Excitatory Amino Acid Transporter 3)
0 (Glutamate Plasma Membrane Transport Proteins)
0 (Slc1a1 protein, rat)
0 (Symporters)
3KX376GY7L (Glutamic Acid)
57716-89-9 (4-O-methyl-12-O-tetradecanoylphorbol 13-acetate)
EC 2.7.11.13 (Protein Kinase C)
NI40JAQ945 (Tetradecanoylphorbol Acetate)
YI7VU623SF (Propofol)
- Publication Date:
Date Created: 20030522 Date Completed: 20030807 Latest Revision: 20190701
- Publication Date:
20221213
- Accession Number:
10.1016/s0304-3940(03)00358-6
- Accession Number:
12759177
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