Novel precursor substrates for polythioesters (PTE) and limits of PTE biosynthesis in Ralstonia eutropha.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 7705721 Publication Model: Print Cited Medium: Print ISSN: 0378-1097 (Print) Linking ISSN: 03781097 NLM ISO Abbreviation: FEMS Microbiol Lett Subsets: MEDLINE
    • Publication Information:
      Publication: 2015- : Oxford Oxford University Press
      Original Publication: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies.
    • Subject Terms:
      Cupriavidus necator/*metabolism ; Polyesters/*metabolism ; Polymers/*metabolism; 3-Mercaptopropionic Acid/metabolism ; Butyrates/metabolism ; Cupriavidus necator/growth & development ; Polyesters/chemistry ; Polymers/chemistry ; Propionates/metabolism
    • Abstract:
      A novel class of biopolymers referred to as polythioesters (PTE) was recently detected when the polyhydroxyalkanoate (PHA) accumulating bacterium Ralstonia eutropha was cultivated in the presence of 3-mercaptopropionic acid (3MP) or 3,3'-thiodipropionic acid (TDP). In this study, 3,3'-dithiodipropionic acid (DTDP) and 3-mercaptovaleric acid (3MV) were identified as two additional precursor carbon sources for in vivo biosynthesis of PTE in R. eutropha. Biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3MP, which contributed 19-25% of cell dry matter, was compared referring to the different precursor substrates. Using DTDP as carbon source, which is probably cleaved into two molecules 3MP, yielded an about 2.3-fold higher molar 3MP content of the copolyester than TDP, which is probably cleaved into only one molecule 3MP. Furthermore, cultivation of R. eutropha in the presence of 3MV resulted in biosynthesis of copolymers consisting predominantly of 3HB with low amounts of 3MV and 3-hydroxyvalerate, each contributing less than 5 mol% of the constituents. In contrast, 4-mercaptobutyric acid could be not incorporated into PHAs, although - as documented in this study - five different strategies, various precursor substrates, R. eutropha and also a recombinant strain of Escherichia coli were employed. Therefore, this study not only extended the range of substrates suitable for PTE biosynthesis and also the range of PTE constituents in R. eutropha, it also demonstrates limits for PTE biosynthesis in this bacterium.
    • Accession Number:
      0 (Butyrates)
      0 (Polyesters)
      0 (Polymers)
      0 (Propionates)
      13095-66-4 (4-mercaptobutyrate)
      3BBK323ED8 (thiodipropionic acid)
      B03TJ3QU9M (3-Mercaptopropionic Acid)
    • Publication Date:
      Date Created: 20030503 Date Completed: 20031027 Latest Revision: 20181130
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/S0378-1097(03)00185-X
    • Accession Number:
      12725926