Targeting of MIST to Src-family kinases via SKAP55-SLAP-130 adaptor complex in mast cells.

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  • Additional Information
    • Source:
      Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE
    • Publication Information:
      Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd.
      Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies.
    • Subject Terms:
      Adaptor Proteins, Signal Transducing*; Carrier Proteins/*metabolism ; Mast Cells/*metabolism ; Phosphoproteins/*metabolism ; src-Family Kinases/*metabolism; Amino Acid Sequence ; Animals ; Carrier Proteins/chemistry ; Humans ; Mast Cells/enzymology ; Mice ; Molecular Sequence Data ; Phosphoproteins/chemistry ; Rats ; Sequence Homology, Amino Acid
    • Abstract:
      MIST (mast cell immunoreceptor signal transducer; also termed Clnk) is an adaptor protein structurally related to SLP-76-family hematopoietic cell-specific adaptor proteins. We demonstrate here that two major MIST-associated phosphoproteins expressed in mast cell lines are SLAP-130 and SKAP55, adaptors known to interact with the Src-homology (SH) 2 domain of Src-family protein tyrosine kinases (PTKs). MIST directly associated with SLAP-130 via its SH2 domain, and collaboration of SLAP-130 with SKAP55 was required for the recruitment of MIST to Lyn. Furthermore, MIST was preferentially recruited to Fyn rather than Lyn, which is regulated by higher affinity binding of SLAP-130 and SKAP55 with the Fyn-SH2 domain than the Lyn-SH2 domain. Our results suggest that the MIST-SLAP-130-SKAP55 adaptor complex functions downstream of high-affinity IgE receptor-associated Src-PTKs in mast cells.
      (Copyright 2003 Federation of European Biochemical Societies)
    • Molecular Sequence:
      GENBANK AB092812
    • Accession Number:
      0 (Adaptor Proteins, Signal Transducing)
      0 (CLNK protein, human)
      0 (Carrier Proteins)
      0 (FYB1 protein, human)
      0 (Fyb protein, mouse)
      0 (Phosphoproteins)
      0 (SKAP1 protein, human)
      0 (Skap1 protein, rat)
      EC 2.7.10.2 (src-Family Kinases)
    • Publication Date:
      Date Created: 20030412 Date Completed: 20030430 Latest Revision: 20200827
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/s0014-5793(03)00234-5
    • Accession Number:
      12681493