Cloning and nucleotide sequence of carbazole catabolic genes from Pseudomonas stutzeri strain OM1, isolated from activated sludge.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Ouchiyama N;Ouchiyama N; Miyachi S; Omori T
  • Source:
    The Journal of general and applied microbiology [J Gen Appl Microbiol] 1998 Feb; Vol. 44 (1), pp. 57-63.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Microbiology Research Foundation Country of Publication: Japan NLM ID: 0165543 Publication Model: Print Cited Medium: Internet ISSN: 1349-8037 (Electronic) Linking ISSN: 00221260 NLM ISO Abbreviation: J Gen Appl Microbiol Subsets: PubMed not MEDLINE
    • Publication Information:
      Publication: Tokyo : Microbiology Research Foundation
      Original Publication: Tokyo, Institute of Applied Microbiology, Univ. of Tokyo.
    • Abstract:
      A new carbazole (CAR)-degrading bacterium, called strain OM1, was isolated from activated sludge obtained from sewage disposal plants in Fukuoka Prefecture, and it was identified as Pseudomonas stutzeri. Anthranilic acid (AN), 2'-aminobiphenyl-2,3-diol and its meta-cleavage product, 2-hydroxy-6-oxo-6-(2'-aminophenyl)-hexa-2,4-dienoic acid, were identified as metabolic intermediates of CAR in the ethyl acetate extract of the culture broth. Therefore, the CAR catabolic pathway to AN in strain OM1 was indicated to be identical to those found in the Pseudomonas sp. strains CA06 and CA10. The strain OM1 degraded catechol (CAT) via a meta-cleavage pathway in contrast to strains CA06 and CA10, which transform catechol into cis, cis-munonic acid. Clones containing a 6.9-kb EcoRI fragment and a 3-kb PstI-SphI fragment were isolated from colonies, forming a clear zone of CAR and a yellow ring-cleavage product from CAT, respectively. Recombinant E. coli carrying the 6.9-kb fragment degraded CAR in the L-broth and produced AN. Cell-free extract from the clone carrying a 3-kb PstI-SphI fragment had high meta-ring-cleavage dioxygenase activity for CAT. The nucleotide sequences of these fragments were determined. The 6.9-kb fragment showed a very high degree of homology with the CAR catabolic genes of strain CA10. The amino acid and nucleotide sequences of the 3-kb fragment were found to exhibit significant homology with the genes for the CAT-catabolic enzymes of TOL plasmid pWW0, plasmid NAH7, and plasmid pVI150.
    • Publication Date:
      Date Created: 20021227 Latest Revision: 20191120
    • Publication Date:
      20240829
    • Accession Number:
      10.2323/jgam.44.57
    • Accession Number:
      12501294