Limited effect of recombinant porcine interleukin-12 on porcine lymphocytes due to a low level of IL-12 beta2 receptor.

Publisher: Elsevier Scientific Country of Publication: Netherlands NLM ID: 8002006 Publication Model: Print Cited Medium: Print ISSN: 0165-2427 (Print) Linking ISSN: 01652427 NLM ISO Abbreviation: Vet Immunol Immunopathol Subsets: MEDLINE

Original Publication: [Amsterdam] Elsevier Scientific.

Interleukin-12/*immunology ; Receptors, Interleukin/*immunology ; Swine/*immunology ; T-Lymphocytes/*immunology; Amino Acid Sequence ; Animals ; Biological Assay ; Blotting, Western ; Cattle ; Cells, Cultured ; Enzyme-Linked Immunosorbent Assay ; Gene Expression ; Humans ; Interferon-gamma/analysis ; Interferon-gamma/biosynthesis ; Interleukin-12/administration & dosage ; Interleukin-12/analysis ; Interleukin-12/genetics ; Lymphocyte Activation ; Molecular Sequence Data ; Protein Subunits ; RNA, Messenger/analysis ; RNA, Messenger/genetics ; Receptors, Interleukin/chemistry ; Receptors, Interleukin/genetics ; Receptors, Interleukin-12 ; Sequence Homology, Amino Acid ; Suipoxvirus/genetics ; T-Lymphocytes/cytology

The cytokine interleukin-12 (IL-12) is a key molecule in the regulation of CD4 + T cell development and specifically potentiates T helper 1 responses in mouse and man. However, biological effects mediated by IL-12 have not been well defined in pigs. Herein, recombinant porcine IL-12 (rPoIL-12) was expressed in a swine poxvirus system as a biologically active heterodimer and used to stimulate bovine or swine lymphoblast cells. After 3 days of incubation, only bovine blasts were responsive to the rPoIL-12 treatment as monitored by cell proliferation in several independent trials. Similarly, i.m. administration of rPoIL-12 in the hind leg of 3-week-old pigs indicated a reduction in the number of interferon-gamma (IFN-gamma) producing lymphocytes isolated from inguinal lymph nodes. The porcine IL-12R beta2 (IL-12Rbeta2) sequence was cloned and results generated by reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that the expression of IL-12R on porcine blasts as measured by the relative levels of IL-12Rbeta2 mRNA was less than that in bovine blasts and are in agreement with the reduced proliferation response of swine blast cells to rPoIL-12 treatment. Real time PCR analysis demonstrated that after PBMC stimulation, bovine blasts had an 11-fold increase in IL-12Rbeta2 mRNA levels while porcine blasts had almost no change. These data support a mechanism for IL-12 stimulation in swine inconsistent with that observed in conventional models.
(Copyright 2002 Elsevier Science B.V.)

Erratum in: Vet Immunol Immunopathol. 2003 Oct 15;95(304):183.

0 (IL12RB2 protein, human)
0 (Il12rb2 protein, mouse)
0 (Protein Subunits)
0 (RNA, Messenger)
0 (Receptors, Interleukin)
0 (Receptors, Interleukin-12)
187348-17-0 (Interleukin-12)
82115-62-6 (Interferon-gamma)

Date Created: 20021018 Date Completed: 20030124 Latest Revision: 20190901

20240829

10.1016/s0165-2427(02)00205-2

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